Molecular characterization of HSP70-1 gene in Beetal goat
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Date
2021-04-15
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Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Abstract
HSP70 play a variety of functions in the cells and are responsible for cytoprotection under stress conditions. The present study was conducted to carry out molecular characterization of HSP70-1 gene in Beetal goat and analyzing genetic diversity through sequences available in the databases. Genomic DNA was extracted from whole blood of Beetal goat. HSP70-1 gene was amplified using specific primers designed by Primer 3 software. The amplified PCR product (1917 bp) was sequenced by Sangers dideoxy chain termination method with “Primer Walking”. A partial cDNA sequence of goat HSP70-1 gene of 1839 bp encoding 613 amino acids was obtained. Comparison of nucleotide and deduced amino acid sequences of Beetal goat HSP70-1 gene was done with different species of animals. The comparison of Beetal goat HSP70-1 partial cDNA sequence demonstrated 99.08, 98.10 and 97.66 percent homology with sheep, buffalo and cattle, respectively which indicates close evolutionary relationship and high sequence homology among the species. Deduced amino acid sequence of 613 residues of Beetal goat HSP70-1 gene was 98.83 and 98.99 percent similar to cattle and buffalo, respectively. The phylogenetic tree drawn by MEGA X software at nucleotide level showed conserved nature of HSP70-1 gene. The pair wise distance between sequences aligned with ClustalW method was estimated by MEGA X software. Maximum divergence from Beetal partial cDNA was observed with chicken CDS and minimum with sheep. Z test based on relative abundance of synonymous and non synonymous substitution indicates purifying selection and not positive selection operating on HSP70-1 gene.