High resolution genetic mapping of xa43(t) gene for bacterial blight resistance in rice (oryza sativa l.)

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dc.contributor.advisorVikal, Yogesh
dc.contributor.authorBhatia, Sukhpreet
dc.date.accessioned2021-02-09T07:41:13Z
dc.date.available2021-02-09T07:41:13Z
dc.date.issued2020
dc.description.abstractBacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the epidemic diseases in rice causes significant yield losses worldwide, especially in Southeast Asia. Due to the lack of effective chemical control, most effective strategy to combat the BB disease is the finding of new resistance sources and its deployment in the elite rice cultivars. Since the durability of resistance is always at stake due to the rapid change in the pathogenicity of the Xoo pathogen, there is a need to identify and characterize novel BB resistance genes. Tagging and fine mapping is pre-requisite for transfer and pyramiding of BB resistance genes through marker-assisted selection (MAS). Therefore, the present investigation was undertaken to develop high resolution genetic map of novel bacterial blight resistance gene, xa43(t) identified from Oryza rufipogon acc. CR100098A. The backcross introgressed lines (BILs, BC1F7) were developed from cross between PR114 and Oryza rufipogon acc. CR100098A. The genetic studies using BC1F2 population and BC1F3 progenies showed single recessive locus conditioning resistance to the Xoo pathotype #7 (PbXo-7). The BILs were segregated in the ratio of 197 resistant : 195 susceptible which is consistent with the expected allelic frequency of 1:1 ratio for single gene inheritance. The xa43(t) gene was tagged with two SSR markers viz. RM27154 and RM2136 on long arm of chromosome 11 using bulked segregant analysis. To fine map the gene, 85 SSR and 86 KASP markers of chromosome 11 were surveyed for parental polymorphism. A total of 22 SSR and five KASP markers were found to be polymorphic between parents. The polymorphic markers were analysed on 392 BILs and genetic analysis placed the xa43(t) BB resistance gene between two flanking SSR markers, PAU11_39 and PAU11_44 within an approximately 447 kb region. Bioinformatic analysis revealed a total of 73 genes within that region and seventeen of them were putative candidate genes involved in biotic stress resistance. The novelty of xa43(t) gene was confirmed by testing the linked markers on other cultivars as well as by surveying the linked markers of other BB resistance genes present on chromosome 11 on parents and bulks.en_US
dc.identifier.citationBhatia, Sukhpreet (2020). High resolution genetic mapping of xa43(t) gene for bacterial blight resistance in rice (oryza sativa l.) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810161057
dc.keywordsRice, Bacterial blight (BB), Xanthomonas oryzae pv. oryzae (Xoo), xa43(t), SSR markers, KASP markers, MASen_US
dc.language.isoEnglishen_US
dc.pages79en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemHigh resolution genetic mapping of xa43(t) gene for bacterial blight resistance in rice (oryza sativa l.)en_US
dc.subAgricultural Biotechnologyen_US
dc.themeHigh resolution genetic mapping of xa43(t) gene for bacterial blight resistance in rice (oryza sativa l.)en_US
dc.these.typeM.Scen_US
dc.titleHigh resolution genetic mapping of xa43(t) gene for bacterial blight resistance in rice (oryza sativa l.)en_US
dc.typeThesisen_US
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