Identification Of Candidate Gene For Genetic Male Sterility Gene, ms-1 IN MUSKMELON (Cucumis melo L.)
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Date
2023
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Punjab Agricultural University
Abstract
Hybrid breeding is preferred in muskmelon as hybrids are uniform, higher yielding and early maturing. Genetic male sterility is one of the most extensively exploited pollination control mechanisms for hybrid breeding in muskmelon. However, a codominant molecular marker is required to rogue out heterozygous male fertile plants in the female line before pollination. Previously, during the molecular mapping of the male sterility ms-1 gene, six putative candidate genes were identified in 400kb region on chromosome six. The present study was conducted for the identification and validation of male sterility ms-1 candidate gene through gene expression analysis and SNP genotyping. Two contrasting parents for the ms-1 trait namely, MS-1 inbred line (male-sterile) and KP4HM-15 inbred line (male-fertile) were used. The flower buds of four different sizes referring to different stages of microspore development were used for gene expression analysis using real time PCR. Of all the six putative candidate genes the expression of PDI gene was significantly higher in male fertile parent KP4HM-15 than male sterile parent MS-1. Insilico analysis also revealed the missense mutation within its protein coding region confirming the PDI gene to be most likely the candidate gene for ms-1. Further, the two parents were used to generate the F2 mapping population. Ten samples each with male-sterile genotype (msms) and male-fertile genotype (MsMs) were pooled separately, to construct male sterile bulk and male fertile bulk respectively. One polymorphic KASP marker was able to distinguish both male sterile and fertile parents and the extreme bulks. The SNP site for this polymorphic KASP marker was present in the exonic region of PDI gene. Thus candidate gene (PDI) and the KASP marker linked to male sterility gene ms-1 were identified and validated which can be used for early selection of male sterile plants.
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Goyal, Aashima (2023). Identification Of Candidate Gene For Genetic Male Sterility Gene, ms-1 IN MUSKMELON (Cucumis melo L.) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.