Optimization of cellulose – andhemicellulose- saccharification from sugarcane bagasse for alcohol fermentation
| dc.contributor.advisor | Nath, T. | |
| dc.contributor.author | Dilip A, Rajput | |
| dc.date.accessioned | 2020-12-18T10:39:05Z | |
| dc.date.available | 2020-12-18T10:39:05Z | |
| dc.date.issued | 2018-01 | |
| dc.description.abstract | Lignocellulosic alcohol production from agricultural residues has the great potential to produce transport fuel as the substitute of petroleum or diesel, as it is renewable in nature, no competition with food as well as less emission problems. Sugarcane bagasse is one of the largest agricultural wastes in many countries including India. The country produces ~40 million metric ton (MMT) of bagasse annually, most of which is burnt and contributes to air pollution. So, it can be considered as one of the lignocellulosic sources for alcohol production, but it needs proper characterization as a feed for alcohol production. In this present research, sugarcane bagasse from Assam (Buraliksion, RARS, AAU, Jorhat) has been tried to be characterized considering its sugar components (cellulose and hemicellulose) which ultimately contributes to ethanol production. Cellulose and hemicellulose composition were found 37.6% and 29.2%, respectively.The process complexity, mainly pretreatment (prior to saccharification) and enzymatic saccharification (conversion to complex carbohydrates to fermentable monomers) has been studied. Among the different pretreatment methods available, the most commonly practiced one, i.e. Acid-Alkali treated method was studied and subsequent enzymatic hydrolysis was done with known commercial enzymes. Hemicellulose was hydrolyzed by hemicellulase enzyme procured from Sigma-Aldrich and found the maximum efficiency of releasing reducing pentose monomers along with acid pretreatment (H2SO4) about 65% of total hemicellulose content. Cellulose was hydrolyzed by cellulase enzyme procured from HiMedia and found the maximum efficiency of releasing reducing hexose sugars (glucose) about 74% of total cellulose content.Not having complete conversion or having less conversion of hemicellulose/cellulose to its monomeric units may be associated with less efficiency of the enzyme or complexity of the cellulose or both. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810156930 | |
| dc.language.iso | English | en_US |
| dc.publisher | AAU, Jorhat | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.theme | Optimization of cellulose – andhemicellulose- saccharification from sugarcane bagasse for alcohol fermentation | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Optimization of cellulose – andhemicellulose- saccharification from sugarcane bagasse for alcohol fermentation | en_US |
| dc.type | Thesis | en_US |
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