GENETIC ANALYSIS OF HOST RESISTANCE TO GASTRO INTESTINAL NEMATODE INFECTION AMONG NATIVE GOAT BREEDS OF KERALA
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Date
2023-03-23
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COLLEGE OF VETERINARY AND ANIMALS SCIENCES MANNUTHY, THRISSUR , KERALA VETERINARY AND ANIMAL SCIENCES UNIVERSITY
Abstract
Present study aimed at analysing the breed variation in host resistance and resilience towards natural gastro-intestinal nematode infection (GIN) and the association of two putative SNPs conferring host resistance in ATP2A3 and SERPING 1 genes was conducted among native goat breeds viz., Attappady Black (n= 51) and Malabari (n= 58) goats of Kerala. The host resistance and resilience were measured using faecal egg count (FEC) and volume of packed red cells (VPRC), respectively. The incidence (per cent) of strongylosis in goats ranged from 87.85 to 100 during the study period of June/ 2021 to October/2021. Larval culture revealed Haemonchus contortus as the most predominant strongyle species. Majority of goats (66.0031 per cent) were very heavily infected with FEC>1500. The correlation among FEC and VPRC was negative (r= -0.409) and highly significant (p<0.01). The genotyping of ATP2A3_680_A>C SNP by polymerase chain reaction– restriction fragment length polymorphism (PCR- RFLP) assay revealed that all the sampled animals were monomorphic for AA genotype. Bi- directional PCR allele specific amplification (Bi-PASA) assay showed the occurrence of all the three genotypes at SERPING1_312_C>T SNP locus. The influence of breed was highly significant (p<0.01) on FEC. The Malabari goats had significantly (p<0.01) lower FEC compared to that of Attappady Black goats. The effects of breed and genotypes of SERPING1_312_C>T SNP on VPRC were statistically significant (p≤0.01). The results indicated that Malabari goats were more resistant towards natural GIN compared to Attappady Black goats under semi-intensive system of rearing.