Molecular mapping of yellow mosaic virus resistance in bitter gourd (Momordica charantia L.)
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Bitter gourd (Momordica charantia) is a popular cultivated vegetable in India and other
countries. The susceptible parent ‘Punjab-14’ and the resistant parent ‘PAUBG-6’ were
crossed to obtain F4 mapping population comprising 101 individuals with the objective of
mapping yellow mosaic disease resistance in bitter gourd. In the present study, genotypingby-sequencing
(GBS) approach was used to develop the genetic linkage map. The map
contained 3,144 single nucleotide polymorphism (SNP) markers, consisted of 15 linkage
groups and spanned for 2415.2 cM with an average marker distance of 0.7 cM. By adopting
the artificial and field inoculation techniques, F4:5 individuals were phenotyped for disease
resistance in Nethouse (2019), Rainy (2019) and Spring season (2020). The QTL analysis
using the genetic map and phenotyping data identified three QTLs qYMD.pau_3.1,
qYMD.pau_4.1 and qYMD.pau_5.1 on chromosome 3, 4 and 5 respectively with phenotypic
variation explained (PVE) from 13.5 to 22.1%. In addition, nine horticultural important traits
including days to appearance of first female flower, days to appearance of first male flower,
days to fruit maturity, node to appearance of first female flower, seed hardness, seed number,
fruit length, fruit diameter and fruit weight were evaluated using quantitative data. As a result,
twelve QTLs responsible for these traits were also identified. In another experiment, the
relative gene expression of begomovirus genes in susceptible and resistant hosts was studied
to understand the mechanism of virus resistance. The expression analysis indicated the high
expression of AC4 gene in resistant plant provided the information on the efforts of virus to
combat the resistance of plant. The information generated in this study is very useful in future
for fine-mapping and for marker-assisted selection for these traits.
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Citation
Gurpreet Kaur (2021). Molecular mapping of yellow mosaic virus resistance in bitter gourd (Momordica charantia L.) (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.