CHEMISTRY AND POTENTIAL OF VETIVER OIL AS STORED GRAIN PROTECTANT
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Date
2013
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PAU Ludhiana
Abstract
The present investigation deals with Chemistry and potential of vetiver oil as stored grain
protectant. Vetiver oil was subjected to column chromatography to have non polar and polar
fractions. Different fractions were subjected to thin layer chromatography in order to find the
number of compounds present in different fractions. Rearing of Tribolium castaneum (Herbst)
adults were carried out. Vetiver oil, its non-polar and polar fractions were tested for their
bioefficacy against adults of Tribolium castaneum by releasing them in wheat grains spiked
with various concentrations of vetiver oil, its non-polar and polar fractions viz. 30,000,
35,000, 40,000, 45,000, and 50,000 μg g-1 respectively. The observations of mortality were
noted every 24 hrs till complete or constant mortality was obtained. The corrected per cent
mortality was calculated using Abbott’s formula. Vetiver oil was found to be effective at all
concentrations tested. The non-polar fraction of petroleum ether was found to be most active
whereas the acetone fraction was least active. The corrected per cent mortality increased with
increase in concentration and time of application. The most active petroleum ether fraction
was subjected to extensive column chromatography to isolate the pure compounds. Khusinol
having melting point 73oC was isolated as pure compound. The compound was characterized
by FT-IR, 1H NMR and 13C NMR spectroscopy and mass spectrometry.
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