Fine mapping of QTL (qSLB8.1 and qSLB9.1) for southern corn leaf blight resistance in maize (Zea mays L.)

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dc.contributor.advisorSharma, Priti
dc.contributor.authorSimranjit Kaur
dc.date.accessioned2021-09-09T06:39:32Z
dc.date.available2021-09-09T06:39:32Z
dc.date.issued2020
dc.description.abstractSouthern corn leaf blight (SLB) caused by Cochliobolus heterostrophus, is a fungal disease of maize. It is prevalent in warm region and can cause yield losses upto 40 percent. Therefore the present investigation was undertaken using 192 RILs developed from cross between LM5 (resistant parent) and CM140 (susceptible parent). RILs along with parental lines were evaluated for SLB disease reaction by inoculating plants at V7-9 stage and data was recorded three times in an interval of 15 days. Disease reaction data showed normal distribution which indicates the quantitative nature of inheritance. Days to anthesis (DTA), days to silking (DTS) and anthesis-silking interval (ASI) were also evaluated and low correlation was observed between flowering traits with disease data. A total of 100 SSR markers of chromosome 8 and 50 SSR markers of chromosome 9 were developed using MISA. Among 221 markers, 71 showed polymorphism between the parental lines. A linkage map was constructed with genomic coverage of 56.3 cM for chromosome 8 (within marker interval umc1075 and bnlg1067) with 20 markers and 9.6 cM for chromosome 9 (within marker interval bnlg1626 and bnlg1091) with 10 markers. QTL analysis was done using QTL cartographer and previously identified QTL qSLB8.1 has been narrowed down with PAU_63-PAU_116 (qSLB8.1) as new flanking markers from distance of 7 cM/110 Mb to 3 cM/42.4 Mb; and in qSLB8.2 distance has been narrowed down to 3.9 cM from 5.6 cM by QTL flanked by markers PAU_95-umc1872 (qSLB8.4). Further, more markers can be designed and identified flanking markers can be used for marker assisted selection.en_US
dc.identifier.citationSimranjit Kaur (2020). Fine mapping of QTL (qSLB8.1 and qSLB9.1) for southern corn leaf blight resistance in maize (Zea mays L.) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810175551
dc.keywordsCochliobolus heterostrophus, SLB, SSR markers, QTL, Zea mays L.en_US
dc.language.isoEnglishen_US
dc.pages70en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemFine mapping of QTL (qSLB8.1 and qSLB9.1) for southern corn leaf blight resistance in maize (Zea mays L.)en_US
dc.subAgricultural Biotechnologyen_US
dc.themeFine mapping of QTL (qSLB8.1 and qSLB9.1) for southern corn leaf blight resistance in maize (Zea mays L.)en_US
dc.these.typeM.Scen_US
dc.titleFine mapping of QTL (qSLB8.1 and qSLB9.1) for southern corn leaf blight resistance in maize (Zea mays L.)en_US
dc.typeThesisen_US
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