Pathological and Molecular Studies on Caprine Respiratory Mycoplasmosis
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Date
2024-04-10
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MAFSU, Nagpur
Abstract
Respiratory mycoplasmosis, is a very severe disease among all
Mycoplasma infections in goats. It shows high mortality and morbidity rates
in the goat population, ultimately responsible for substantial economic losses
to the farmers. This study aimed to describe pathomorphology and molecular
characterization of Mycoplasma spp. causing respiratory mycoplasmosis in
caprines. Out of 790 screened animals, total of 155 pneumonic lung tissue
samples were collected from the vicinity of Akola, Maharashtra. All the
samples were subjected to DNA extraction followed by PCR, using
Mycoplasma genus specific primers. Out of 155 samples 54 (34.8%) samples were positive for genus Mycoplasma with specific amplicon size of 280 bp
by PCR. Subsequently, Mycoplasma genus positive samples were subjected
to M. ovipneumoniae and Mccp identification by using species specific
primers. Among 155 caprine lung samples, 39 (25.16%), 15 (9.6%) and 6
(3.87%) samples were positive for M. ovipneumoniae, Mccp and concurrent
infection, respectively. Phylogenetic analysis of Mycoplasma isolates from
the current study confirmed the isolates as M. ovipneumoniae and Mccp.
Histopathological studies of Mycoplasma positive lung tissue samples
revealed various types of pneumonia include, bronchopneumonia, interstitial
pneumonia, bronchointerstitial pneumonia, with the most characteristic
lesions such as fibrinous pleuritis, bronchitis with BALT hyperplasia,
bronchial and bronchiolar epithelial hyperplasia, necrotic alveolitis with type
II pneumocytic hyperplasia, thickened interalveolar and interstitial septae,
syncytial cell formation, goblet cell hyperplasia, and marked infiltration of
inflammatory exudate rich in neutrophils and mononuclear cells. Present
study warrants to conduct large scale molecular studies for understanding the
concept of patho-epidemiology of mycoplasmosis in goats to implement
preventive strategies to minimize production losses in farmers.