Synergistic effect of Mesorhizobium and non rhizobial endophytes on plant growth promotion in chickpea (Cicer arietinum L.)

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dc.contributor.advisorSharma, Poonam
dc.contributor.authorChhabra, Deepika
dc.date.accessioned2018-05-11T11:16:36Z
dc.date.available2018-05-11T11:16:36Z
dc.date.issued2018
dc.description.abstractThe present investigation was carried out to exploit non rhizobial endophytic bacteria from root and nodules of chickpea. Of 263 non rhizobial endophytic bacteria 74.4 %, 87.4%, 12.6%, 36.6%, 37.4% and 55.7% were found to be positive for catalase, oxidase, citrate utilization, nitrate reduction, methyl red and Voges Proskauer‟s, respectively. 71 were found to be good for phosphate solubilization and IAA production. Significantly high P solubilization was observed in root isolate RBR20 (20.60 mg100ml-1) whereas, among nodule‟s isolates with RBN17 (21.0 mg100ml-1). In the presence of tryptophan, the isolate LCRE 9 produced the maximum amount of IAA (39.60 μgml-1) whereas in the absence of tryptophan the isolate RBR164 produced the maximum amount of IAA (19.93 μgml-1). RBN16 isolate showed highest growth in DF medium with ACC (OD 1.04) followed by RBR164 (OD 0.9985). High amount of GA production was observed in RBR19, RBR127, RBR136, RBR164 and LCNE6 (112.15 μgml-1). Highest amount of EPS production was observed LCRE8 (105.01 μgml-1). Out of 75 isolates 38.7%, 82.7%, 9.3% and 21.3% isolates were able to produce cellulase, protease, HCN and fluorescent pigment, respectively. On the basis of overall PGP traits and compatibility studies 3 potential non rhizobial endophytic bacteria (RBR164, LCRE9 and RBN17) were selected for colonization study in different combinations with ampicillin (6μgml-1) an antibiotic marker. Colonization was maximum in RB1+LGR33+ RBN164 treatment (1.16 and 1.60) at 15th and 50th respectively. Triple inoculant treatment LGR33+RB1+RBR164 resulted into maximum increase in plant height, dry weight of shoot and total bacterial count at 90 DAS as compared to dual, single and uninoculated control under field conditions. Maximum increase in dry weight of root, root shoot ratio, nodule dry weight and leghaemoglobin was observed with same treatment at 60 and 90 DAS. Similar trend was observed for total NPK content of shoot and seed. Grain yield was increased with triple inoculants treatment LGR33+RB1+RBR164 by 8.9% and 3.6% over uninoculated control and recommended consortium of chickpea, respectively. It seems from the foregoing study that consortium of Mesorhizobium with multifarious plant growth promoting endophytic bacteria can be developed and used as biofertilizer for chickpea.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810045355
dc.keywordsChickpea, Colonization, Endophytes, Cicer arietinum L.en_US
dc.language.isoenen_US
dc.pages146en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemSynergistic effect of Mesorhizobium and non rhizobial endophytes on plant growth promotion in chickpea (Cicer arietinum L.)en_US
dc.subMicrobiologyen_US
dc.subjectnullen_US
dc.themeSynergistic effect of Mesorhizobium and non rhizobial endophytes on plant growth promotion in chickpea (Cicer arietinum L.)en_US
dc.these.typePh.Den_US
dc.titleSynergistic effect of Mesorhizobium and non rhizobial endophytes on plant growth promotion in chickpea (Cicer arietinum L.)en_US
dc.typeThesisen_US
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