MOLECULAR AND PHYSIOLOGICAL ANALYSIS OF TRANSGENIC RICE HARBOURING CHIMERIC PDH47 GENE AGAINST ABIOTIC STRESS TOLERANCE

Simple item page
dc.contributor.advisorSingh, Salvinder
dc.contributor.authorBoro, Dimple
dc.date.accessioned2021-02-05T11:01:17Z
dc.date.available2021-02-05T11:01:17Z
dc.date.issued2020-02
dc.description.abstractAbiotic stress especially drought can severely affect the physiological status of any plants; thereby impart significant negative impact on growth, development, and metabolism. The major emphasis of most of the recent studies was identification of stress-regulated genes and transcription factors which play important role in governing tolerance/resistance against abiotic stresses. DEAD-box helicases (Asp-Glu-Ala-Asp amino acid) are one of the important genes which confer tolerance to various abiotic stresses. They are involved in unwinding of nucleic acids by utilizing the energy from ATP hydrolysis. PDH47 (Pea DNA Helicase 47kDa) gene, one of the DEAD-box helicases is known to impart various abiotic stress tolerance. In the present study rice transgenic line cv. IR64 was developed through Agrobacterium mediated genetic transformation using immature embryos as explants. The putative transgenic lines showed presence of transgene when subjected to PCR analysis using gene specific primers. Three previously developed transgenic rice lines in our laboratory namely ASD16-46/1, ASD16-66/1 and ASD16-68/1 expressing PDH47 gene were selected for drought stress tolerance study. Quantitative Real Time PCR analysis showed varied level of expression of PDH47 gene both in the root and leaf tissues of transgenic line before and after drought stress. The expression of PDH47 gene induced during drought stress in the transgenic lines, showed varied level of drought tolerance in the vegetative stage without any negative effects on the morphological and agronomical traits. The physiological and biochemical analyses confirmed that the expression of PDH47 gene in the transgenic lines was associated with increased leaf relative water content, water retention capacity, maintenance of chlorophyll, stomatal conductance, net photosynthetic rate, transpiration rate and water use efficiency. These transgenic lines also showed an increased accumulation of the osmolytes like proline, glycine betaine and decreased electrolyte leakage, lipid peroxidation, less accumulation of H2O2 during drought stress. These transgenic lines showed better root architecture system such as root length, root number, root thickness, root biomass, shoot biomass and root to shoot ratio as compared to control untransformed plants during drought stress. The western blot analysis confirmed that the expression of RUBISCO large subunit (55kDa) protein was more in the transgenic rice lines as compared to untransformed control plants during drought stress treatment.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810160933
dc.language.isoEnglishen_US
dc.publisherAAU, Jorhaten_US
dc.subAgricultural Biotechnologyen_US
dc.themeMOLECULAR AND PHYSIOLOGICAL ANALYSIS OF TRANSGENIC RICE HARBOURING CHIMERIC PDH47 GENE AGAINST ABIOTIC STRESS TOLERANCEen_US
dc.these.typePh.Den_US
dc.titleMOLECULAR AND PHYSIOLOGICAL ANALYSIS OF TRANSGENIC RICE HARBOURING CHIMERIC PDH47 GENE AGAINST ABIOTIC STRESS TOLERANCEen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
MOLECULAR AND PHYSIOLOGICAL ANALYSIS OF TRANSGENIC RICE HARBOURING CHIMERIC PDH47 GENE AGAINST ABIOTIC STRESS TOLERANCE.pdf
Size:
3.08 MB
Format:
Adobe Portable Document Format
Description:
Ph.D Thesis
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses