Pretreatment of paddy straw for cultivation of Vplvariella (Bull.ex Fr.) Singer
| dc.contributor.advisor | Sodhi, Harpreet Singh | |
| dc.contributor.author | Swarthi K. | |
| dc.date.accessioned | 2019-11-12T05:28:29Z | |
| dc.date.available | 2019-11-12T05:28:29Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Two strains of Volvariella volvacea, VV3 and VV6 were evaluated for their mycelia growth rate and yield potential on pretreated paddy straw as substrate under Punjab conditions. Mycelial growth studies on Nutrient agar (NA) and Potato Dextrose agar (PDA) inoculated with Bacillus sp. and Delftia sp. separately revealed that bacteria had no antagonistic effect on the growth of V. volvacea. The mycelia growth rate of V. volvacea on PDA inoculated with bacterium was statistically at par with control and covered plate in 5 days whereas on NA mycelia growth covered the plate in 8 days. The effect of pretreatment of paddy straw on its chemical composition was studied with 6 different pretreatments: urea 1% (w/w), urea 0.5% (w/w), urea 0.5% (w/w)+Delftia sp. 0.5% (v/w), urea 0.5% (w/w) +Bacillus 0.5% (v/w), Delftia sp. 0.5%, Bacillus sp. 0.5% (v/w) and water 0.5% (v/w) as control. The pretreated paddy straw was analysed for proximate principles at a periodic interval of 5 days upto 20 days. Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), Cellulose, hemicelluloses, Acid Detergent Lignin (ADL), Ash content and crude protein content were analysed. A significant decline in NDF value from 75.7% to 46 % and 34.4% was observed when urea 0.5% + Bacillus 0.5% + Delftia 0.5% were used as pretreatment of paddy straw respectively.The ADF value was significantly reduced to 23% and 33% when urea 0.5% +Delftia 0.5% and urea 0.5% + Bacillus 0.5% were used to treat paddy straw. A significant reduction in cellulose content was observed from 40% to 25% to 20% with urea 0.5%+ Delftia 0.5% and urea 0.5% + Bacillus 0.5% respectively. The hemicelluloses content recorded maximum reduction to 11.45% from 23.4% with urea 0.5% + Delftia 0.5%. There was a rapid decline in ADL with urea 0.5% + Delftia 0.5% and urea 0.5% + Bacillus 0.5% from 12.3% to 4.3% and 7% respectively. The ash and crude protein content increased with all the pretreatments. Studies on mycelia growth rate on pretreated paddy straw revealed that the addition of bacteria along with urea had improved the growth rate of both the strains of V. volvacea indicating the significant role of bacterial pretreatment in the mycelia growth of V.volvacea. Cultivation of V.volvacea strains on pretreated paddy straw had shown the maximum yield was obtained from pretreated paddy straw from VV6 (14.84 kg/ q dry straw) followed to strain VV3 (13.82 kg/q dry straw) on pretreated paddy straw. The yield from untreated (control) paddy straw was 10.5 kg/q dry straw from VV3, 12.5 kg/q dry straw from VV6. Present study revealed that pretreatment of paddy straw with urea 0.5%+Delftia sp. 0.5% gave better yield than untreated paddy straw. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810135081 | |
| dc.keywords | Volvariella, paddy straw, pretreatment , lignin, cultivation. | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 49 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Pretreatment of paddy straw for cultivation of Vplvariella (Bull.ex Fr.) Singer | en_US |
| dc.sub | Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | Pretreatment of paddy straw for cultivation of Vplvariella (Bull.ex Fr.) Singer | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Pretreatment of paddy straw for cultivation of Vplvariella (Bull.ex Fr.) Singer | en_US |
| dc.type | Thesis | en_US |