EVALUATION AND MOLECULAR CHARACTERIZATION OF LEAF AND STRIPE RUST OF WHEAT
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Date
2024-05-13
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Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu
Abstract
Leaf rust and Stripe rust of wheat are responsible for significant losses in yield potential.Although, chemical control of thesediseasesisknownbutitisnoteconomicandenvironmentally friendly.Inpresentinvestigation wasduetofrequent breakdown of major seedling resistance genes in recent years and difficulties in quick replacement of susceptible wheat varietiesleadstoinvestigatesofotherformofdiseaseresistancelikeadultplantresistance(APR)andslowrustingwhich are considered asmore durable. Out of 96 germplasm and three susceptible checks viz.,Agra local, HD2967, PBW343 for stripe rust,Agra local, Karachia-65and Lalbahadur for leaf rust, nine germplasm GWJ39, GWJ48, GWJ50, GWJ53, GWJ55, GWJ64, GWJ66, GWJ77 and GJW83 for stripe rust, ten germplasm viz., GWJ38, GWJ39, GWJ56, GWJ57, GWJ61,GWJ75,GWJ77,GWJ88,GWJ89andGWJ90forleafrustshowedimmuneresponsebothatseedlingstageand adultplantstage.Seventeengermplasmviz.,GWJ5,GWJ6,GWJ10,GWJ18,GWJ19,GWJ34,GWJ35,GWJ36,GWJ37, GWJ38, GWJ45, GWJ52, GWJ54, GWJ59 GWJ69, GWJ89 and GWJ91 showed resistant reaction in seedling stage against stripe rust pathotypes but these lines become susceptible at adult plant stage. Eleven germplasm viz., GWJ3, GWJ6,GWJ33,GWJ51,GWJ54,GWJ55,GWJ69,GWJ76,GWJ81,GWJ83,GWJ84,showedseedlingresistant
reaction against leaf rust pathotypes but these lines become susceptible at adult plant stage. Forty-one germplasm were observedwithACIvaluebetween>0to10asresistantatadultplantstagetostriperust,fifty-sixgermplasmwiththeACI value ranges between >0 to 10 to leaf rust.ACI values between >10 to 20 was recorded in fifteen germplasm for stripe rust and 23 germplasmfor leaf rust consideredasslowrustier line.Molecularcharacterization ofeight Yrgenelike Yr2, Yr5, Yr9, Yr10,Yr15, Yr16, Yr18 and Yr29andthreeLr genelike Lr32, Lr50, Lr67. Yr5 withACI valuein between 0to 5 in thirteen germplasm with frequency of 13.5%. Yr10 race specific withACI range between 0-5 postulated in twelve germplasm with frequency of 12.5 showed resistance response. Yr15 withACI range between 0-5 was postulated in 22 germplasm with frequency of 22.2%. Yr16 non -Race specific ACI value 6 to 12 showed Moderately susceptible to moderatelyresistantresponse and postulatedintwenty germplasmwith20.08%frequency. Yr18 withACIvalue6to60 showed moderately resistance to moderate susceptible response in thirty-two germplasm with 33.33 % frequency and Yr29 non-race specific with ACI value 6 to 60 showed moderately susceptible to susceptible response with 20.8 % frequency. Yr9 was postulated in seventeen germplasm with andACI value ranges between 6 to 40 showed susceptible response with 27.4 % frequency. Yr2 ACI value ranges between 9 to 70 showed susceptible response with in in forty germplasmwith40.4%frequencyforstriperust.RacespecificandallstageleafrustresistantgeneLr32ACIvalueranges between 0 to 3 showed resistance response in six germplasm with 7.29 % frequency. non -Race specific and adult plant resistancegeneLr50withACIvalueranges1to30with23.9%frequency.Anon-Racespecificandadultplantresistance geneLr67withACIvaluewaspostulatedintwenty-germplasmwithvalue1to21showedresistanttomoderateresistant with20.8%frequencytoleafrust.InIndia,prevalentpathotypesforyellowrustinclude46S119,110S119,and238S119. Yr5, Yr10, Yr15, YrSP, and YrSkgenesare resistant toPstpathotypesin Indian conditions, while in the case of leaf rust of wheat, prevalent pathotypes are 77-5, 77-9, and 104-2. Lr9, Lr19, Lr24, Lr25, Lr29, Lr32, Lr39, Lr45, and Lr47 are the genes having resistance to Ptrpathotypes in Indian conditions.
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Ravishankar, L.V. 2024. EVALUATION AND MOLECULAR CHARACTERIZATION OF LEAF AND STRIPE RUST OF WHEAT, P.hD. Thesis, SKUAST-Jammu, India