QUANTIFICATION OF RESISTANCE AND CHARACTERIZATION OF SOYBEAN GENOTYPES IN RESPONSE TO CHARCOAL ROT.

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dc.contributor.advisorNandanwar, Dr. R.S
dc.contributor.authorCHAVAN, SNEHAL VINAY
dc.date.accessioned2020-03-12T10:20:16Z
dc.date.available2020-03-12T10:20:16Z
dc.date.issued2019-06-10
dc.descriptionSoybean being one of the important crop has got a huge scope for development. There is great potential for expansion of this crop in entire Indian plateau by creating disease resistant varieties. Charcoal rot has the potential to reduce the yield more than 70%. Therefore, development of soybean varieties resistant to charcoal rot disease is an important breeding objective and need of the hour. To facilitate breeding, it is necessary to evaluate the soybean germplasm for levels of resistance, as there is not a single known source of true resistance. In order to select germplasm for its use in breeding activities, thorough knowledge about its genetic diversity is important. Different molecular markers (SSR and RGA) can also prove to be important for development of charcoal rot resistant variety of soybean in future.en_US
dc.description.abstractThe present study entitled “Quantification of resistance and characterization of soybean genotypes in response to charcoal rot.” was carried out at Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during the year 2018-19 to evaluate various genotypes of soybean for charcoal rot resistance. Charcoal rot disease caused by Macrophomina phaseolina is one of the most damaging diseases of soybean resulting to 70 % losses and till date no immune genotype is known for the same. Various limitations inherent in field experiments may have hindered progress toward identifying new source(s) of resistance and breeding resistance in soybean. Non-field testing under semi-controlled conditions in a greenhouse or in controlled conditions in plant growth chambers may compliment or help to overcome the limitations of field-testing. The present investigation aimed to establish in-vitro screening procedure for screening of soybean for charcoal rot resistance and identify the resistant genotype(s). Fourteen core genotypes comprising popular varieties, newly released and mutant genotypes were screened under field and in-vitro conditions. The genotypes were screened under sick-plot during Kh-2018 and in-vitro screening was carried out using blotter-paper, pot-inoculation technique, stem cut inoculation, in-vitro on leaf technique using virulent pure culture of M.phaseolina. Disease infection was scored using the scales as per the experiment and genotypes were categorized accordingly. Four genotypes including varieties viz., AMS MB 5 -18, AMS MB 5-19, AMS 77 and AMS 1001 were identified as resistant to M phaseolina in all the in-vivo and in-vitro screening. Molecular characterization of these genotypes was done by using SSR and RGA markers. Molecular profiles revealed remarkable polymorphism and observations showed that in total 181 amplicons were tested with an average of 6.03 alleles per locus. Out of the total screened alleles 62 were monomorphic alleles with an average of 2.07 and 119 were polymorphic alleles with an average of 3.97. Results showed an average of 65.75 polymorphism percent. The PIC (Polymorphic information content) value of 30 microsatellite loci ranged from 0.29 to 0.83 with an average value of 0.69, these studies will help in mapping studies and breeding program for development of charcoal rot resistance in soybean genotypes which will be of utmost importance.en_US
dc.identifier.citationCHAVAN, SNEHAL VINAY (2019). Quantification of resistance and characterization of soybean genotypes in response to charcoal rot. Department of Agricultural Botany (Genetics and Plant Breeding), Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2019. xiii, 118p. (Unpublished).en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810145304
dc.keywordsSoybean, Pulse crop, Biotic stress resistance, Charcoal rot, Resistance breeding, Blotter-paper, Pot-inoculation, Stem-cut inoculation, In-vitro technique, Field evaluation, Molecular studies, Single Sequence Repeats markers, SSR markers, Resistance Gene Analogs markers, RGA markers,en_US
dc.language.isoenen_US
dc.pagesxiii,118p.en_US
dc.publisherDr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.en_US
dc.research.problemIn the present study the researcher has tried to study differential response of soybean core genotypes to artificial infection with charcoal rot incidence. And also tried to study the biochemical and molecular characterization of soybean genotypes differing for charcoal rot resistance reaction.en_US
dc.subBotanyen_US
dc.subjectCrops, Field crops, Glycine max, Soybean, Plant protection, Control methods, Host plant resistance, Crop improvement, Genetics, Genetic techniques, Plant Breeding, Natural selection, Biotechnology, Electrophoresis, Molecular biology, Genetic markers, In-vitro techniques, PCR, Plant Physiology, Carbohydrates, Oils, Proteins,en_US
dc.themeThe present research is based on biotic stress management and evaluation of genetic variability and diversity in disease reaction among soybean genotypes using molecular markers.en_US
dc.these.typeM.Scen_US
dc.titleQUANTIFICATION OF RESISTANCE AND CHARACTERIZATION OF SOYBEAN GENOTYPES IN RESPONSE TO CHARCOAL ROT.en_US
dc.typeThesisen_US
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Soybean being one of the important crop has got a huge scope for development. There is great potential for expansion of this crop in entire Indian plateau by creating disease resistant varieties. Charcoal rot has the potential to reduce the yield more than 70%. Therefore, development of soybean varieties resistant to charcoal rot disease is an important breeding objective and need of the hour. To facilitate breeding, it is necessary to evaluate the soybean germplasm for levels of resistance, as there is not a single known source of true resistance. In order to select germplasm for its use in breeding activities, thorough knowledge about its genetic diversity is important. Different molecular markers (SSR and RGA) can also prove to be important for development of charcoal rot resistant variety of soybean in future.
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