Disease resistance in the management of cowpea aphid-borne mosaic virus
| dc.contributor.advisor | Umamaheswaran, K | |
| dc.contributor.author | Radhika, N S | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2020-11-26T09:11:10Z | |
| dc.date.available | 2020-11-26T09:11:10Z | |
| dc.date.issued | 1999 | |
| dc.description | P G | en_US |
| dc.description.abstract | Investigations were undertaken on the virus causing severe mosaic on cowpea (Vigna unguiculata (L.) Walp) in Kerala. The characteristic symptoms appeared as vein clearing, light and dark green mottling, severe mosaic, dark green vein banding, blistering, distortion and reduction in leaf size. The virus was mechanically transmitted through sap extracted in 0.01 M phosphate buffer (pH 7.0). The virus was efficiently transmitted by the aphid vector, Aphis craccivora. Seed transmission of eleven per cent was recorded in the variety Sharika. Thermal inactivation point was recorded at a range of 60 - 65° C, dilution end point at a range of 10-3 - 10-4 and longevity in vitro for four hours at room temperature (28 ± 4° C) and six hours under refrigerated condition (8° C). A. craccivora could efficiently transmit the virus with an acquisition access of ten minutes and inoculation access of one minute. Pre-acquisition starvation increased the rate of transmission while post-acquisition starvation decreased the rate. A single aphid was capable of transmitting the virus. The virus causing severe mosaic was identified as blackeye cowpea mosaic virus by ELISA. The virus could also be detected by Ouchterlony immunodiffusion test. Electron microscopic studies revealed the presence of flexuous, filamentous particles of 750 nm in length. Two varieties Co-6 and Cc-Selection were grouped as no symptom producing among 65 genotypes screened for resistance. Fifty three F2 progenies of the cross Sharika and Co-6 and twenty five F2 progenies of the cross Co-Selection and Sharika were long poded and resistant. Biochemical changes indicated a lower carbohydrate content in resistant compared to susceptible. Chlorophyll content decreased in the susceptible variety due to virus infection. Increase in protein was observed in both resistant and susceptible. The phenol content did not show variation between the varieties. Peroxidase, polyphenol oxidase and phenylalanine ammonia-lyase activities increased in the resistant variety. Bioassay of chemicals and neem oil on local lesion host (c. amaranticolor) indicated a per cent inhibition of 68.92 by neem oil in pre-inoculation application and 65.45 per cent inhibition by manganese chloride in post-inoculation application. On cowpea plants, pre-inoculation application of neem oil (ten per cent) concentration was found to be effective in reducing the symptoms due to viral infection. | en_US |
| dc.identifier.citation | 171466 | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810155808 | |
| dc.keywords | Cowpea Aphid-Borne Mosaic Virus | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Department of Plant Pathology, College of Agriculture, Vellayani | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | Cowpea Aphid-Borne Mosaic Virus | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Disease resistance in the management of cowpea aphid-borne mosaic virus | en_US |
| dc.type | Thesis | en_US |