Effect of some biotic and abiotic factors on the endospore adherence and development of bacterial parasite, Pasteuria penetrans on meloidogyne javanica
Loading...
Date
2011
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
Influence of plant root exudates, and culture filtrates (CFs) of rhizobacteria and fungi on the
attachment of Pasteuria penetrans endospores to J2 of Meloidogyne javanica was studied in lab
experiments. Soil leachate did not affect the endospore attachment. Endospore attachment increased
significantly with time. Brinjal, tomato, chickpea and onion root exudates enhanced endospore
attachment. Garden bean root exudates promoted the growth and multiplication of protozoans that
decreased the number of leftover endospores drastically. CFs of Azotobacter chroococcum,
Gluconoacetobacter diazotrophicus and Providentia rettgeri promoted, while that of Enterobacter
cloacae reduced the endospore attachment to J2 of M. javanica. The CFs (autoclaved or not) of soil
fungi adversely affected the endospore attachment to nematode juveniles. Studies on attempted in vitro
culture of P. penetrans in CFs of rhizobacteria revealed the growth of P. penetrans-like stages (tetrads,
diads etc.) only in case of A. chroococcum, E. cloacae and G. diazotrophicus. In a screen-house
experiment to study the development of P. penetrans during rabi season, it was observed that the life
cycle of host nematode M. javanica was prolonged; although eggs were formed in the genital tracts of
females but no egg masses were formed till the end of March. The sporogenesis phase of P. penetrans
did not occur in any of the five sets inoculated on different dates. P. penetrans development was
restricted to vegetative phase only (microcolonies and thallus stages). The nematode development
continued albeit slowly; while that of bacterium failed to synchronize with that of nematode.
Description
Keywords
Sowing, Vegetative propagation, Germinability, Biological development, Biological phenomena, Drying, Developmental stages, Genetics, Attitude, Biomass