Role of plant growth promoting microbial consortium as bioprotectant for controlling Fusarium wilt in chickpea (Cicer arietinum L.)
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
The present investigation was undertaken to develop a plant growth promoting microbial bioinoculant as biofertilizer and bioprotectant to reduce the Fusarium wilt in chickpea (Cicer arietinum L.) for improving crop productivity in sustainable manner. Mesorhizobium (15) and endophytic bacterial (5) isolates were assessed for antagonism against wilt causing Fusarium oxysporum sp. ciceris. Mesorhizobium (6) and endophytic bacterial (5) isolates were selected as potential antagonists on the basis of fungal growth inhibition ranged from 50-93.7%. Compatible consortium of Mesorhizobium sp. and endophytic bacteria was developed by plate assay and broth studies, further screened for multifarious PGP traits viz. IAA, Phosphate-solubilization, siderophore, ACC-deaminase and seed germination in single and dual treatments. Maximum IAA (50.13μg/ml) and siderophore production (4.0cm) was recorded with LGR33+RB-1. Highest P-solubilization (13.93mg/100ml) and ACC-deaminase (OD600nm 1.492) was observed with LGR2+NE8. Mesorhizobium (LGR33 and LGR2) and all endophytic isolates were able to grow on recommended dose of captan in disc plate method. Synergistic effect of Mesorhizobium sp. and endophytic bacteria was evaluated for biocontrol, growth, symbiotic parameters and grain yield in chickpea (desi PBG-7 and PBG-1) under wilt sick field. Significant improvement in growth and symbiotic parameters was observed with LGR2+NE8 (69NN/plant, 104.24mg nodule dry weight, leghaemoglobin content of 5.13μg/g nodules) as compared to control. An enhanced antioxidant enzymes viz. catalase (15.04U/min/g FW), peroxidase (2.404U/min/g FW) and total phenols (109.54 tannic acid equivalents/g FW) were observed with the appearance of disease in chickpea roots. On pooled mean basis dual inoculant LGR2+LCNE8 improved the grain yield by 6.5% over control. It thus appears that the consortium of Mesorhizobium sp. and endophytic bacteria can be explored as potential bioinoculant for control of wilt incidence in chickpea.
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