Assessment of hybrid purity in sunflower by using molecular markers
| dc.contributor.advisor | Ghodke, M.. K. | |
| dc.contributor.author | Bhosle, Sheetal Madhukarrao | |
| dc.date.accessioned | 2018-06-18T05:17:31Z | |
| dc.date.available | 2018-06-18T05:17:31Z | |
| dc.date.issued | 2011-08-16 | |
| dc.description.abstract | Sunflower (Helianthus annum L.) being a cross pollinated crop, genetic adulteration is vested problem and varietal description is cumbersome. For screening genetically pure hybrids RAPD and SSR marker were used for fingerprinting of hybrids, assessing variation within parental lines and testing the genetic purity of ten hybrids. Among 29 primers nine RAPD and seven SSR primers found polymorphic across the hybrids and produced unique fragment for ten hybrids. Four set of RAPD and SSR markers OPC 16, OPA 11, OP-03, OPA 02 and Ha 1327, ORS 5, ORS 662, ORS 243 respectively differentiated most of the hybrids. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped the hybrids into five clusters. Within the clusters most of the hybrids showed a common cytoplasmic male sterile line as female parent and restorer line as male parent. According to presence and absence of bands that RAPD and SSR markers were classified into five groups. Bands commonly showed by in parents and hybrids included in marker pattern I and III found significant in respect to the hybrid confirmation test. The bands from parents does not appear in hybrids include in II, IV and V marker pattern. The parental bands expressed uniquely in hybrid and individual in marker type so it has direct implication in identification of hybrids and can be useful in protecting the rights of plant breeders. SSR marker was found more significant over RAPD in hybrid purity assessment | en_US |
| dc.identifier.other | T06209 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810053628 | |
| dc.keywords | Hybrid, Polymorphism, Orthogonal, RAPD, Molecular, Hybrid, Genetic Purity, fingerprinting | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 120 | en_US |
| dc.publisher | Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | AGRICULTURE | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Assessment of hybrid purity in sunflower by using molecular markers | en_US |
| dc.type | Thesis | en_US |