DIFFERENTIAL FOLLICULAR MORPHOLOGY AND BMPR1B GENE EXPRESSION IN MALABARI AND ATTAPPADY BLACK BREEDS OF GOATS
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Date
2015
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
Prolificacy difference between two native goat breeds of Kerala, Malabari and
Attappady black has been evaluated by morphometric, histological, hormonal and gene
expression studies. Six numbers each of Malabari and Attappady black goat breeds were
used for the study. Even though there was no significant difference, the morphometric
parameters (weight, length and breadth) recorded were more in Malabari than Attappady
black breed of goats. The follicular development was studied by classifying the follicles
based on size into very small (<1mm), small (1-3mm) and large (3-5mm). The total
number of follicles was more in Malabari when compared to Attappady black. However,
follicles classified under small size were more in Attappady breed of goats whereas,
follicles under very small size and large size were more in Malabari. Haematoxylin and
eosin stained sections were examined for histological studies and the different categories
of follicles namely primordial, primary, secondary and antral were compared between
breeds. The diameter of secondary follicle and the oocyte were significantly different
with a higher mean value for Attappady breed. The ratio of granulosa cell to secondary
follicle diameter and the ratio of oocyte to secondary follicle diameter were found to be
significantly different between the breeds with higher mean in Malabari breed. The mean
diameter of the antrum in Attappady breed was higher than Malabari breed. The ratio of
granulosa cell to the antral follicle diameter was not significantly different between
breeds. The serum oestrogen concentration was found to be significantly different
between the breeds with Malabari having a higher mean (± S.E) 41.69 ± 7.50 pg/mL
when compared to the Attappady breed with a mean (± S.E) 20.20 ± 4.40 pg/mL. There
was no significant difference in the BMPR1B gene expression level between breeds.
However, there was significant down regulation of BMPR1B expression in large follicles
compared to small ones.
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