Growth Hormone Gene Polymorphism and Its Association with Growth Performance in Deccani Sheep
| dc.contributor.advisor | Bankar, P. S. | |
| dc.contributor.author | Bhavekar, A. P. | |
| dc.date.accessioned | 2023-04-10T11:08:49Z | |
| dc.date.available | 2023-04-10T11:08:49Z | |
| dc.date.issued | 2022-01-17 | |
| dc.description.abstract | The present research was conducted with the objectives to study the genetic polymorphism of Growth hormone gene using PCR-RFLP as well as to investigate the association of polymorphic genotypes with growth performance in Deccani sheep in the Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, PGIVAS, Akola. In the present study, growth performance (body weight) and important morphometric traits (Body length, height at wither, chest girth and Paunch girth) were recorded in 63 Deccani sheep in Yavatmal district, belonging to both sexes and different age groups. The growth performance and morphometric traits in 32 lambs were systematically recorded from birth to 3 months of age, at 15 days intervals each. The body weight and respective morphometric traits in randomly selected 31unrelated ewe and ram belonging to 6, 9 and 12 months of age were recorded once during a visit to herds. To screen the polymorphism of GH gene (422 bp), total 32 Deccani lambs from different herds and sexes were considered. The DNA was collected from the blood using blood genomic DNA isolation kit. The reported primer of GH gene (422 bp) with Forward (5’CTCTGCCTGCCCTGGACT3’) and Reverse primer: (5’ GGAGAAGCAGAAGGCAACC 3’) was used to amplify the selected locus. After the digestion of HaeIII restriction enzyme, the samples executed three different genotypes, which were assigned on the basis of bands, screened through gel electrophoresis. The present study revealed polymorphic pattern for GH gene i.e. distinct restriction conformational patterns indicating a total of three genotypes AA (366, 56 bp), AB (422,366 and 56 bp) and BB (422 bp) were observed. The highest genotypic frequency (0.72) was noted for AB genotype as compared to BB (0.16) and AA (0.12) genotypes with a gene frequency of A and B allele as 0.48 and 0.52, respectively for GH gene in studied Deccani sheep. The findings indicated that the B allele was more frequent than the A allele among the studied Deccani population for the GHgene locus (422 bp). The data on growth performance traits in Deccani lambs viz. body weight and morphometric traits measured periodically was subjected for Least Squares Mean estimation. The overall least squares mean for body weight (kg) at birth, 1 month (30 days), 2 months (60 days), 3 months (90 days) in Deccani lambs were 2.72±0.67, 9.86±0.49, 14.19±0.57 and 19.32±0.63 kg, respectively. The mean values observed for body weight (kg) at 6, 9 and 12 months of age were 27.38±0.44, 32.04±1.00, and 37.89±1.80 kg, respectively. The present investigation revealed, body length at birth, 1, 2, 3, 6, 9 and 12 months of age months estimates (cm) were 32.72±1.05, 42.86±0.97, 51.71±1.48, 60.41±1.6, 65.1±1.28, 65.3±1.61 and 78.06±0.58 cm respectively. Similarly, the estimates (cm) for height at wither were 38.86±0.93, 48.24±1.28, 56.30±1.52, 64.30±1.58, 61.8±1.04, 62.9±0.69, and 74.16±0.77, respectively; the estimates (cm) for chest girth were 40.11±1.06, 47.62±1.13, 54.59±1.27, 62.61±1.20 74.4±1.35, 74.6±0.87 and 78.91±1.51, respectively and the estimates (cm) for paunch girth were 38.54±0.88, 45.72±0.98, 53.11±1.01, 59.90±1.04, 78.95±2.93, 78.3±1.39 and 78.19±1.17, respectively. The effect of sex, herd and genotype were investigated as sources of variation. The effect of sex was highly significant (p<0.01) source of variation for body weight at birth only. The effect of herd was found non significant source of variation at all ages except for body length at one month of age (p<0.05) in Deccani lambs. However, the effect of sex at other stages as well as effect of herd and genotypes at all stages were found as non significant sources of variation for body weight and morphometric traits in studied population of Deccani lambs. In present investigation, the body weight and morphometric traits studied in both sexes at different ages were indicating that Deccani sheep as mutton purpose breed. The present study suggested that, there was requirement for assessment of other loci and genes for the association of growth traits in Deccani sheep. | en_US |
| dc.external.URL | www.mafsu.in | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810196247 | |
| dc.keywords | Animal Genetics, Animal Breeding, Growth Hormone, Gene, Polymorphism, Deccani Sheep | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 109 | en_US |
| dc.publisher | MAFSU, Nagpur | en_US |
| dc.sub | Animal Genetics and Breeding | en_US |
| dc.theme | Growth Hormone Gene Polymorphism and Its Association with Growth Performance in Deccani Sheep | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | Growth Hormone Gene Polymorphism and Its Association with Growth Performance in Deccani Sheep | en_US |
| dc.type | Thesis | en_US |