STUDY OF HORMONES AND BIOCHEMICAL CONSTITUENTS OF FOLLICULAR FLUID OF UNOVULATED FOLLICLES AND OVARIAN TISSUE IN SUPEROVULATED GOATS
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Date
2001
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AAU, Anand
Abstract
Totally 24 indigenous goats of Gujarat state were superovulated with Folltropin-V (FSH) and FolHgon (PMSG) during low and peak breeding season of the year to study the hormonal and biochemical profile of follicular fluid of unovulated follicle, luteal tissue and ovarian interstitial tissue of superovulated goats. The goats were mated on the day of superovulatory estrus with an injection of 750 lU of chorulon (LH) to facilitate maximum ovulation and were laparotomized on day-3 of the superovulatory estrus. The superovulatory response was recorded in terms of number of ovulation and number of unovulated follicles. The ovaries were removed surgically and after recording the biometrical changes, 10 % tissue homogenates of luteal and interstitial tissue were prepared in distilled water. The follicular fluid, luteal tissue homogenate and ovarian interstitial tissue honragenate were analyzed for Hormones (progesterone, estradiol-17 p and testosterone), biochemical (total protein and total, free and ester cholesterol), enzymes (AKP, ACP and LDH) and micro minerals (copper, iron and zinc) by standard procedures. The results revealed that the mean ovarian length (21.76 ± 1.89 Vs 13.20 ± 1.06 mm), width (16.04 ± 1.77 Vs 9.74 ± 0.69 mm) and thickness (12.01 ± 1.14 Vs 7.66 ± 0.62 mm) were significantly (P<0.05) higher in superovulated goats than the control one. The differences in these parameters between the two superovulatory drugs were statistically non-significant. The superovulatory response in terms of mean number of ovulation (12.83 ± 2.58 Vs 8.91 ± 1.90) and mean number of embryo recovery (5.91 ±2.14 Vs 3.66 ± 1.11) was recorded significantly (P<0.05) higher in folltropin-v treated animals as compared to folligon treated animals. However, the mean number of unovulated follicles did not differ significantly between the two treatment groups (3.50 ± 0.90 and 3.75 ± 1.65 respectively for folltropin-v and folligon treated animals). The effect of season on superovulatory response was statistically non-significant. The studies on follicular fluid of unovulated follicles indicated that the progesterone, estradiol 17- (3 and testosterone concentration did not differ significantly between the treated groups. The effect of season was also non-significant specifically for estradiol 17-P and testosterone but progesterone was significantly (P<0.05) high during low breeding season. The total protein content was recorded higher in follicular fluid of superovulated goats compared to control (14.47 ±1.20 Vs 12.87 ± 1.12 mg %). The cholesterol concentration was lower in follicular fluid of unovulated follicles compared to control animals but the differences were statistically non-significant. The season also did not influence the cholesterol content in follicular fluid. The AKP and LDH activity was slightly higher in control animals than treated groups. The ACP activity was significantly (P<0.05) higher in PMSG and FSH treated animals than control (2.84 ± 0.20 Vs 3.10 ± 0.16 Vs 2.31 ± 0.15 KAU%). The effect of season was statistically non-significant for AKP, ACP and LDH activity in follicular fluid of unovulated follicles.
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REPRODUCTIVE BIOLOGY, A STUDY