MOLECULAR DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN CHICKEN

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dc.contributor.advisorSurya Sankar
dc.contributor.authorNIRANJANA S. RAJALAKSHMI
dc.date.accessioned2020-09-23T08:30:18Z
dc.date.available2020-09-23T08:30:18Z
dc.date.issued2019
dc.description.abstractAvian infectious bronchitis, an acute highly contagious disease of chickens, affects birds of all age groups. The present study envisaged the isolation, identification and molecular characterisation of IBV. For the study, samples were collected from chickens of different ages with signs of respiratory tract infection. Out of the 95 samples collected, 42 were positive for IBV using specific UP and DOWN primers targeting the highly conserved 5’UTR region. The samples were inoculated into nine to eleven day-old embryonated chicken eggs via., allantoic cavity. Virus inoculated embryos after six passages exhibited the characteristic curling and dwarfing. The harvested allantoic fluid revealed the presence of IBV by RT-PCR in 42 samples. Reverse transcriptase polymerase chain reaction identified IBV in three more allantoic fluid. A Taqman probe based real time PCR was developed targeting the highly conserved 5’UTR and N genes which detected 45 positives in suspected tissue samples and allantoic fluid. The hypervariable regions 1, 2 and 3 of spike gene of all the positive samples were amplified by RT-PCR using three different primers and the respective amplicons were sequenced to study the genetic diversity of the isolates. The sequences of the isolates were compared with those of commercial vaccine strain (H120) and other Indian isolates available in NCBI GenBank database. All the sequences showed more than 95 per cent similarity with the commercial vaccine strain as well as with the Indian isolates from various regions. Phylogenetic tree was constructed based on the obtained sequences. On phylogenetic analysis of hypervariable regions 1 and 2, all the four Thrissur isolates clustered with each other and also with the IBV isolates from different regions. Whereas, on phylogenetic analysis of hypervariable region 3, all the four Thrissur isolates were branched separately among themselves and also with the IBV isolates from different regions of the country. Thus, a comprehensive analysis of these variations could aid in the selection of a suitable candidate vaccine strain.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151838
dc.keywordsMOLECULAR DETECTION INFECTIOUS BRONCHITIS VIRUS CHICKENen_US
dc.language.isoEnglishen_US
dc.pages93en_US
dc.publisherCollege of Veterinary and animal Science,Mannuthyen_US
dc.research.problemMOLECULAR DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN CHICKENen_US
dc.subVeterinary Microbiologyen_US
dc.themeMOLECULAR DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN CHICKENen_US
dc.these.typeM.V.Sc.en_US
dc.titleMOLECULAR DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN CHICKENen_US
dc.typeThesisen_US
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