COMPARATIVE EFFICACY OF EGG YOLK AND SOYA BASED EXTENDERS FOR REFRIGERATION (5 °C) AND CRYOPRESERVATION (-196 °C) OF BUFFALO SEMEN

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dc.contributor.advisorDhami, A. J.
dc.contributor.authorCHAUDHARI, DINESHKUMAR V.
dc.date.accessioned2018-06-21T10:41:26Z
dc.date.available2018-06-21T10:41:26Z
dc.date.issued2014
dc.description.abstractThe present investigation was undertaken during the favourable breeding season (November-February) of the year 2013-2014 on six mature Surti buffalo bulls at Central Sperm Station of Department of Gynaecology and Obstetrics of the Veterinary College, AAU, Anand. The study covered evaluation of seminal characteristics in neat semen and then comparative efficacy of egg yolk based standard TFYG (Tris-citric acid-fructoseegg yolk-glycerol) extender and soybean based commercially available extenders (Bioxcell® and Optixcell®, IMV, France) using split-ejaculate technique through various morphological and functional attributes of spermatozoa extended/preserved/ processed in these extenders for refrigeration preservation (at 5°C up to 72 hrs) and cryopreservation (-196°C), including interrelationships of quality sperm parameters of fresh, refrigerated and cryopreserved semen. Immediately after collection, the ejaculates (8 per bull) were evaluated for routine physico-morphological attributes, including motility, viability, morphology (eosinnigrosin), acrosomal integrity (Giemsa stain) and plasma membrane integrity (HOST 150 mOsm/L; an in vitro fertility test) through standard procedures and using phase contrast microscope.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810054913
dc.keywordsCOMPARATIVE EFFICACY OF EGG YOLK, SOYA BASED EXTENDERS, REFRIGERATION (5 °C), CRYOPRESERVATION (-196 °C) OF BUFFALO SEMENen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemCOMPARATIVE EFFICACY OF EGG YOLK AND SOYA BASED EXTENDERS FOR REFRIGERATION (5 °C) AND CRYOPRESERVATION (-196 °C) OF BUFFALO SEMENen_US
dc.subAnimal Reproduction and Gynecologyen_US
dc.subjectANIMAL REPRODUCTION, GYNAECOLOGY AND OBSTETRICSen_US
dc.subjectCOMPARATIVEen_US
dc.themeCOMPARATIVE EFFICACY OF EGG YOLK AND SOYA BASED EXTENDERS FOR REFRIGERATION (5 °C) AND CRYOPRESERVATION (-196 °C) OF BUFFALO SEMENen_US
dc.these.typeM.V.Sc.
dc.titleCOMPARATIVE EFFICACY OF EGG YOLK AND SOYA BASED EXTENDERS FOR REFRIGERATION (5 °C) AND CRYOPRESERVATION (-196 °C) OF BUFFALO SEMENen_US
dc.typeThesisen_US
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