COMPARATIVE EFFICACY OF EGG YOLK AND SOYA BASED EXTENDERS FOR REFRIGERATION (5 °C) AND CRYOPRESERVATION (-196 °C) OF BUFFALO SEMEN
dc.contributor.advisor | Dhami, A. J. | |
dc.contributor.author | CHAUDHARI, DINESHKUMAR V. | |
dc.date.accessioned | 2018-06-21T10:41:26Z | |
dc.date.available | 2018-06-21T10:41:26Z | |
dc.date.issued | 2014 | |
dc.description.abstract | The present investigation was undertaken during the favourable breeding season (November-February) of the year 2013-2014 on six mature Surti buffalo bulls at Central Sperm Station of Department of Gynaecology and Obstetrics of the Veterinary College, AAU, Anand. The study covered evaluation of seminal characteristics in neat semen and then comparative efficacy of egg yolk based standard TFYG (Tris-citric acid-fructoseegg yolk-glycerol) extender and soybean based commercially available extenders (Bioxcell® and Optixcell®, IMV, France) using split-ejaculate technique through various morphological and functional attributes of spermatozoa extended/preserved/ processed in these extenders for refrigeration preservation (at 5°C up to 72 hrs) and cryopreservation (-196°C), including interrelationships of quality sperm parameters of fresh, refrigerated and cryopreserved semen. Immediately after collection, the ejaculates (8 per bull) were evaluated for routine physico-morphological attributes, including motility, viability, morphology (eosinnigrosin), acrosomal integrity (Giemsa stain) and plasma membrane integrity (HOST 150 mOsm/L; an in vitro fertility test) through standard procedures and using phase contrast microscope. | en_US |
dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810054913 | |
dc.keywords | COMPARATIVE EFFICACY OF EGG YOLK, SOYA BASED EXTENDERS, REFRIGERATION (5 °C), CRYOPRESERVATION (-196 °C) OF BUFFALO SEMEN | en_US |
dc.language.iso | en | en_US |
dc.publisher | AAU, Anand | en_US |
dc.research.problem | COMPARATIVE EFFICACY OF EGG YOLK AND SOYA BASED EXTENDERS FOR REFRIGERATION (5 °C) AND CRYOPRESERVATION (-196 °C) OF BUFFALO SEMEN | en_US |
dc.sub | Animal Reproduction and Gynecology | en_US |
dc.subject | ANIMAL REPRODUCTION, GYNAECOLOGY AND OBSTETRICS | en_US |
dc.subject | COMPARATIVE | en_US |
dc.theme | COMPARATIVE EFFICACY OF EGG YOLK AND SOYA BASED EXTENDERS FOR REFRIGERATION (5 °C) AND CRYOPRESERVATION (-196 °C) OF BUFFALO SEMEN | en_US |
dc.these.type | M.V.Sc. | |
dc.title | COMPARATIVE EFFICACY OF EGG YOLK AND SOYA BASED EXTENDERS FOR REFRIGERATION (5 °C) AND CRYOPRESERVATION (-196 °C) OF BUFFALO SEMEN | en_US |
dc.type | Thesis | en_US |
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