ISOLATION AND CHARACTERIZATION OF Staphylococcus aureus FROM MILK AND MILK PRODUCTS
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Date
2010
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Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
In the present study altogether 190 samples, comprising 100 samples of raw
milk and 90 samples of milk products were examined.50 samples each of raw
milk were taken from two different sources i.e farms and private vendors and 30
samples each of peda, kalakand and paneer were collected from local shops and
standard shops of Ranchi city and were subjected to Staphylococcal count and
result was expressed in log10 scale.
30 samples of peda, comprising 15 samples taken from local shops and 15
from standard shops showed Staphylococcal count ranging from 0.000 to 5.397
with an average of 2.011±0.585 log10/g and 0.000 to 4.477 with an average of
1.379±0.470 log10/g respectively.Similarly 30 samples of kalakand, comprising 15
samples taken from local shops and 15 from standard shops showed
Staphylococcal count ranging from 0.000 to 7.845 with an average of
3.170±0.855 log10/g and 0.000 to 7.344 with an average of 3.076±0.894 log10/g
respectively.Staphylococcal count in 30 samples of paneer, comprising 15
samples from local shops and 15 samples from standard shops ranged from
0.000 to 8.701 with an average of 4.832±0.923 log10/g and 0.000 to 7.049 with an
average of 3.893±0.855 log10/g respectively.
In 100 samples of raw milk, comprising 50 samples collected from farms and
50 samples from private milk vendor the Staphylococcal count ranged from 0.000
to 7.819 With an average of 2.820 ± 0.411 log10/g and 0.000 to 9.049 with an
average of 3.862±0.470 log10/g respectively.
The recovery percentage of Staphylococcus spp from peda samples collected
from local and standard shops was 53.84% and 50.00% respectively. Similarly
percentage of Staphylococcus from kalakand samples from local and standard
shops was 57.14% and 53.84% and that of paneer samples was recorded as
90.90% and 90.00% respectively.The isolation percentage of Staphylococcus
spp from milk samples collected from farms and private milk vendors was
recorded as 59.5% and 62.5% respectively.
102 isolates of Staphylococcus spp were isolated out of which 9 strains were
of Coagulase negative Staphylococci and 93 strains were of Coagulase positive
Staphylococci. Out of 93 strains of Coagulase positive Staphylococcus, 91
strains were Staphylococcus aureus and 2 were other species of
Staphylococcus. Incidence of S.aureus was lower in peda, kalakand and paneer
collected from standard shops than local shops.The contamination in paneer
samples was higher followed by kalakand and peda collected from both local and
standard shops.
Out of 93 strains of Coagulase positive Staphylococcus spp isolated from milk
and milk products, 91 Staphylococcus aureus strains were subjected to biotyping
on the basis of three properties-hydrolysis of Tween 80, pigmentation on Tween
80 Agar and Urease production. It was found that majority of isolates fell in
biotype A (46.15%) followed by D (32.96%), B (10.98%) and C (09.89%).
All the 102 isolates of Staphylococcus were subjected to in vitro drug
sensitivity test using 10 different antimicrobial agents i.e Gatifloxacin,
Sparfloxacin, Ciprofloxacin, Ofloxacin, Azithromycin, Aztreonam, Doxycycline,
Vancomycin, Ampicillin and Cotrimoxazole.The drug sensitivity test revealed that
Staphylococcus spp were highly sensitive to Gatifloxacin followed by Ofloxacin,
Ciprofloxacin and Sparfloxacin , moderately sensitive to Vancomycin,
Doxycycline, Aztreonam and Azithromycin. The organisms were resistant to
Ampicillin and Cotrimoxazole.
Results indicate that strict preventive measures should be adopted to ensure
contamination free milk products for good health of consumers.
Description
ISOLATION AND CHARACTERIZATION OF Staphylococcus aureus FROM MILK AND MILK PRODUCTS
Keywords
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