ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF LISTERIA SPECIES FROM MARKET MILK
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Date
2010
Authors
Journal Title
Journal ISSN
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Publisher
Anand Agricultural University, Anand
Abstract
The aim of the present study was to isolate, identify and characterize
Listeria spp. from milk and milk products sold at and around Anand city, Gujarat
State.
A total of 237 samples comprising 147 raw milk (68 Cow milk and 79
buffalo milk), 38 pasteurized milk, and 52 milk products (12 Curd, 11 Ice cream,
12 Butter milk, 9 Lassi, 8 Cheese) samples were collected aseptically from
various organized (branded) as well as unorganized (unbranded) sectors
supplying milk and milk products to consumers at and around Anand city and
subjected to two-step enrichment in University of Vermont broth and Frasers
broth , followed by plating on three selective media viz. PALCAM, LPM agar
and Oxford media. The presumptive isolates were identified at genus and species
level by the battery of biochemical tests. These isolates were further subjected to
in vitro pathogenicity (hemolysis on sheep blood agar, CAMP test and
Phosphatidylinositol-specific phospholipase
Description
Keywords
Veterinary Public Health, Study