Simultaneous saccharification and fermentation of sugarcane bagasse for bioethanol production
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Date
2012
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Publisher
CCSHAU
Abstract
With the ever increasing demand for energy and the fast depleting petroleum resources, the
interest in alternative fuels, especially liquid transportation fuels, has been steadily increasing all over
the world .The ethanol is a superior automotive fuel as its molecule contains oxygen which allows
almost complete combustion of the fuel by the engine, resulting in fewer emissions. Since ethanol is
produced from plants that harness the power of the sun, ethanol is also considered a renewable fuel.
Current production of bioethanol in India relies on molasses, but there has been considerable debate
about its sustainability and hence there is greater interest in second generation fuels utilizing cheaper
lignocellulosic materials as a feedstock for ethanol production. Among lignocelluloses, sugarcane
bagasse has immense potential as a renewable substrate. Sugarcane bagasse does not compete with
food crops and is also less expensive than conventional agricultural feedstocks. Non milled sugarcane
bagasse procured from sugarcane mill, Meham (Rohtak) contained 31.7% cellulose, 23.7%,
hemicellulose and 22.8% lignin. Treatment with 5% NaOH for 1 hour was found to be best treatment in
terms of change in composition of substrate and time of pretreatment. There was 75.9% decrease in
lignin content whereas 28.0% increase was observed in cellulose. Maximum ethanol production
(3.1%) was observed when simultaneous saccharification and fermentation of 5% alkali pretreated
milled bagasse was carried out with Saccharomyces cerevisiae in buffered nutrient solution at substrate
loading of 10% and enzyme loading 30FPU/g of the substrate.
Description
Keywords
Yields, Animal husbandry, Livestock, Lactation, Genetics, Breeds (animals), Sire evaluation, Seasons, Biological phenomena, Phenotypes