CHARACTERISATION OF GENES AFFECTING METABOLIC ADAPTATION IN TRANSITION DAIRY COWS

Simple item page
dc.contributor.advisorShynu M.
dc.contributor.authorVASUDHAR BHAT S.V.
dc.date.accessioned2020-09-23T08:17:34Z
dc.date.available2020-09-23T08:17:34Z
dc.date.issued2019
dc.description.abstractThe period from three weeks before parturition until three weeks after parturition is the transition period in dairy cows. This time period is critical in the health and production of dairy cows and profitability of dairying as most diseases occur during this period. The dairy cows undergo several metabolic adaptations to overcome the stress of foetal growth and lactation. Even when maintained under similar feeding and managemental conditions, dairy cows exhibit variation in adaptability. This indicates the genetic influence on metabolic adaptability. The present study was performed in 30 apparently healthy pregnant crossbred dairy cows with an objective to characterize three genes responsible for metabolic adaptability and to detect variations in these genes, among animals with varying adaptability, as indicated by their BHBA concentration. Blood was collected and serum was separated at fortnightly intervals from four weeks before the predicted day of parturition until four weeks after parturition. Concentration of NEFA and BHBA were determined. The mean concentration of NEFA (0.254±0.21 mmol/L) and BHBA (0.608±0.024 mmol/L) during pregnancy were significantly elevated in comparison with the NEFA (0.349±0.37 mmol/L) and BHBA (0.681±0.23 mmol/L) during lactation. As BHBA is a better indicator of metabolic adaptability, the animals were grouped based on their BHBA concentration using cluster analysis. The genes DNAJC30, SNAI2 and UEVLD known to influence metabolic adaptability were sequenced from representative animals of each group. Four differences (transitions) in sequence were observed downstream the coding region of DNAJC30 gene between the groups. One change was located in the promoter region and another in exon one of SNAI2 gene. The CDS of UEVLD gene did not show any change when compared with the available Bos taurus sequence in NCBI. The variations in genes studied might contribute to difference in the metabolic adaptability between the groups. Further studies in a larger population, is required to ascertain the suitability of using these as markers for selection of dairy animals for better metabolic adaptabilityen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151834
dc.keywordsCHARACTERISATION GENES AFFECTING METABOLIC ADAPTATION TRANSITION DAIRY COWSen_US
dc.language.isoEnglishen_US
dc.pages84en_US
dc.publisherCollege of Veterinary and animal Science,Mannuthyen_US
dc.research.problemCHARACTERISATION OF GENES AFFECTING METABOLIC ADAPTATION IN TRANSITION DAIRY COWSen_US
dc.subAnimal Biochemistryen_US
dc.themeCHARACTERISATION OF GENES AFFECTING METABOLIC ADAPTATION IN TRANSITION DAIRY COWSen_US
dc.these.typeM.V.Sc.en_US
dc.titleCHARACTERISATION OF GENES AFFECTING METABOLIC ADAPTATION IN TRANSITION DAIRY COWSen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
VASUDHAR BHAT S.V..pdf
Size:
3.07 MB
Format:
Adobe Portable Document Format
Description:
CHARACTERISATION OF GENES AFFECTING METABOLIC ADAPTATION IN TRANSITION DAIRY COWS
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thesis