EFFICACY OF GREEN SYNTHESISED ZINC OXIDE NANOPARTICLES AGAINST MULTI-DRUG RESISTANT NON- TYPHOIDAL SALMONELLA SPP.

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dc.contributor.advisorJess Vergis
dc.contributor.authorVARSHA UNNI
dc.date.accessioned2023-04-05T06:10:16Z
dc.date.available2023-04-05T06:10:16Z
dc.date.issued2022-04-02
dc.descriptionSubmitted in partial fulfilment of the requirement for the degree of Master of Veterinary Science in Veterinary Public Healthen_US
dc.description.abstractIn the recent wake of antimicrobial resistance, nanotechnology has received remarkable attention and has been considered as a promising interventional tool for treating the drug resistant pathogens. The present study evaluated the antibacterial efficacy of green synthesised Zinc Oxide nanoparticles (ZnO NPs) against multi-drug resistant non-typhoidal Salmonella spp. (MDR-NTS). The synthesis and characterisation of ZnO NPs was attempted using the aqueous extract of Piper longum catkin. Initially, the characterisation of green synthesised ZnO NPs was performed by UV- Vis spectroscopy, Fourier transform infra- red spectroscopy (FTIR), Thermogravimetric analysis (TGA) and differential thermogravimetric analysis (DTA), powder X-ray diffraction (PXRD), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM). The green synthesised ZnO NPs exhibited an absorbance peak at 340 nm by UV- Vis spectroscopy, which was confirmed by FTIR analysis. The TGA/DTA revealed a progressive thermal degradation of the ZnO NPs between 250oC and 400oC, however, a good thermal stability was exhibited for annealing temperatures between 900oC and 1300oC. The ZnO NPs exhibited a hexagonal wurtzite crystalline structure by PXRD analysis, which was further confirmed by SEM and TEM. This study evaluated the in vitro antibacterial efficacy of ZnO NPs against MDR- NTS strains (S. enterica Typhimurium and S. Enteritidis (n= 3 for each serotype). The minimum inhibitory concentration (MIC; 125 μg/ml) and minimum bactericidal concentration (MBC; 250 μg/ml) of ZnO NPs was determined by employing microbroth dilution technique. Later, ZnO NPs was also tested for its stability (high- end temperatures, physiological concentration of cationic salts, proteases and pH); safety (chicken RBCs; HEK cell lines) and effect on gut beneficial lactobacilli (Lactobacillus acidophilus and L. plantarum). In general, the ZnO NPs tested stable at MIC concentration; however, a three to four- fold rise in the MBC value was observed. Besides, ZnO NPs were tested safe with chicken RBCs and HEK cell lines at MIC (1X, 2X, 5X and 10X) levels; moreover, the beneficial gut lactobacilli were not inhibited. Furthermore, the in vitro time- kill kinetic assay of MDR-NTS strains treated with ZnO NPs revealed a complete clearance after 360 min. To conclude, the biosynthesised ZnO NPs was found to exhibit antibacterial activity against the tested MDR-NTS, found stable and safe. Overall, the study demonstrated facile, eco- friendly method for the synthesis of ZnO NPs, which could be employed as a potential antimicrobial alternative candidate.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810196074
dc.keywordsEFFICACY OF GREEN SYNTHESISED ZINC OXIDE NANOPARTICLES AGAINST MULTI-DRUG RESISTANT NON- TYPHOIDAL SALMONELLA SPP., EFFICACY OF GREEN SYNTHESISED ZINC OXIDE, NANOPARTICLES AGAINST MULTI-DRUG RESISTANT, NON- TYPHOIDAL SALMONELLA SPP., Salmonellosis, Clinical manifestations , Non typhoidal salmonellosis (NTS) , Antimicrobial resistance (AMR), AMR among NTS strains , Nanotechnology as an alternative therapeutic approach, Metal oxide NPs, Green synthesis and characterisation of NPs, Green synthesis and characterisation of ZnO NPsValidation of MDR-NTS isolates, Antibiotic susceptibility testing ,en_US
dc.language.isoEnglishen_US
dc.pages141 p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES POOKODE, LAKKIDIen_US
dc.research.problemSynthesis and characterization of green synthesized ZnO NPs, Antibacterial activity of green synthesized ZnO NPs against MDR- NTS.en_US
dc.subVeterinary Public Healthen_US
dc.themeEFFICACY OF GREEN SYNTHESISED ZINC OXIDE NANOPARTICLES AGAINST MULTI-DRUG RESISTANT NON- TYPHOIDAL SALMONELLA SPP.en_US
dc.these.typeM.V.Sc.en_US
dc.titleEFFICACY OF GREEN SYNTHESISED ZINC OXIDE NANOPARTICLES AGAINST MULTI-DRUG RESISTANT NON- TYPHOIDAL SALMONELLA SPP.en_US
dc.typeThesisen_US
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