CHARACTERIZATION OF PATHOGENS ASSOCIATED WITH RHIZOME ROT OF GINGER (Zingiber officinale Rosc.) AND DECIPHERING HOST RESISTANCE

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dc.contributor.advisorN. BASAVARAJA
dc.contributor.advisorHEGDE, N. K.
dc.contributor.advisorM. P. BASAVARAJAPPA
dc.contributor.advisorK. C. KIRAN KUMAR
dc.contributor.advisorMULLA, S. R.
dc.contributor.authorMESTA, R. K.
dc.date.accessioned2023-11-06T15:08:36Z
dc.date.available2023-11-06T15:08:36Z
dc.date.issued2021-01-29
dc.description.abstractThe present study was conducted to characterize the pathogens involved in the causation of rhizome rot of ginger and to decipher the resistance mechanism. The study revealed that rhizome rot of ginger is a complex disease caused by the interaction of different pathogens. Isolation of pathogens collected from major ginger growing areas of Karnataka and Kerala revealed the association of pathogens viz; Pythium aphanidermatum, Fusarium oxysporum, Fusarium solani, Sclerotium rolfsii, Ralstonia solanacearum, Meloidogyne hapla and Meloidogyne enterolobii in causing rhizome rot of ginger. Pathogenicity of the individual causal organisms was proved by using Koch postulates. Molecular characterization and species level identification of fungi and bacteria was carried out by using species specific primers. Sequencing of the nematode DNA revealed higher similarity with Meloidogyne hapla and Meloidogyne enterolobii. All the pathogens can cause potential disease as individuals and in combinations at different plant growth stages tested viz; 75 DAP, 105 DAP and 135 DAP. At 135 days after planting plants recorded higher per cent disease incidence and rhizome rot incidence. Combination of P.aphanidermatum, Fusarium spp., Sclerotium rolfsii, Ralstonia solanacearum , Meloidogyne spp. recorded higher per cent disease incidence and rhizome rot incidence in all the intervals. The maximum disease incidence was seen in sequential inoculation of Meloidogyne spp. followed by R. solanacearum. None of the elicitors except biocontrol agents found effective during in vitro evaluation. External application of elicitors resulted in higher expression of defense genes such as ERF transcdription factor, HMGR, HMGS, HSP, ABC, MLO, WRKY, Callose synthase, Glucanase and PR-5. All the genes were expressed in higher fold as compared with zero hours. External application of methyl jasmonate as prophylactic manner resulted in reduced per cent disease incidence and rhizome rot as compared to curative treatments. Screening of varieties under different conditions revealed that none of the varieties showed resistance against the pathogens. Among two different nanoparticles used against the pathogens, copper nanoparticles were found more effective against the disease complex. Antibiotics and fungicides were evaluated under in vitro conditions and the best molecules were evaluated under field conditions during 2018-19 and 2019-20. An IDM module was developed considering all the effective molecules and the treatment having soil application of carbofuran (25 kg /ha), rhizome treatment with COC (3g/L) and streptocycline (0.5 g/L) for 30 min, foliar application methyl jasmonate (200 ppm), drenching with fosetyl (2g/L) followed by drenching with sectin (2g/L) was found most effective in reducing the disease and rhizome rot with highest yield /ha with maximum B: C. 223 ±ÀÄAp (fAf§gï D¦ ü¹£Éïï) A
dc.identifier.otherUHS17PGD218
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810200465
dc.keywordsHorticulture
dc.keywordsPathology
dc.keywordsPlant Pathology
dc.keywordsPathogens
dc.keywordsCausation
dc.keywordsRhizome
dc.keywordsGinger
dc.keywordsDecipher
dc.keywordsMechanism
dc.keywordsIsolation
dc.keywordsPythium Aphanidermatum
dc.keywordsFusarium Oxysporum
dc.keywordsFusarium Solani
dc.keywordsSclerotium Rolfsii
dc.keywordsRalstonia Solanacearum
dc.keywordsMeloidogyne Hapla
dc.keywordsMeloidogyne Enterolobii
dc.keywordsPathogenicity
dc.keywordsFungi
dc.keywordsBacteria
dc.keywordsInoculation
dc.keywordsElicitors
dc.keywordsBiocontrol Agents
dc.keywordsCallose Synthase
dc.keywordsGlucanase
dc.keywordsJasmonate
dc.keywordsProphylactic
dc.keywordsScreening
dc.keywordsNanoparticles
dc.keywordsIn Vitro
dc.keywordsMolecules
dc.keywordsCarbofuran
dc.keywordsStreptocycline
dc.keywordsMethyl Jasmonate
dc.keywordsAntibiotics
dc.keywordsUHS17PGD218
dc.keywordsT702
dc.language.isoEnglish
dc.pages238p. (Due to pdf. conversion there is a difference in pages of soft copy and hard copy).
dc.publisherUniversity of Horticultural Sciences, Bagalkot. (College of Horticulture, Bagalkot).
dc.subPlant Pathology
dc.themeCHARACTERIZATION OF PATHOGENS ASSOCIATED WITH RHIZOME ROT OF GINGER (Zingiber officinale Rosc.) AND DECIPHERING HOST RESISTANCE
dc.these.typePh.D
dc.titleCHARACTERIZATION OF PATHOGENS ASSOCIATED WITH RHIZOME ROT OF GINGER (Zingiber officinale Rosc.) AND DECIPHERING HOST RESISTANCE
dc.typeThesis
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