Expression analysis for virulence causing gene of Bipolaris sorokiniana
| dc.contributor.advisor | Kumar, Mithilesh | |
| dc.contributor.author | Deuri, Priyanka | |
| dc.date.accessioned | 2020-02-10T10:29:50Z | |
| dc.date.available | 2020-02-10T10:29:50Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Wheat [Triticum aestivum (L.) is a most important cereal crop belonging to the tribe Triticeae of the Poaceae family. Wheat is not only staple food but has a increasing global demand for wheat based product. Spot blotch of wheat, caused by Bipolaris sorokiniana (Sacc.) Shoemaker (syn. Helminthosporium sativum teleomorph: Cochliobolus sativus), is a serious constraint to wheat production (Triticum aestivum L.). The pathogen is seed and soil borne. Pusa in Bihar is considered as a hot spot for the disease. Fourteen fungal isolates were collected from infected leaves and seeds of different wheat genotypes grown at the research farms of DRPCAU pusa, TCA Dholi campus, BISA, IARI Regional Station at Pusa .They were characterized morphologically on the basis of colony colour, growth pattern and exudation, and expression analysis of virulence gene ToxA and melanin biosynthesis genes PKS1 and SCD1 on six different morphological groups of isolates. The isolates were divided into six morphological groups on the basis of their colony colour namely-black, grey, brownish black, greenish black, olive and dull white, among which the frequency of black was the maximum on natural papulation. To examine the relative gene expression among the isolates, RNA was isolated then first strand c-DNA synthesized by reverse transcription process. The RT-PCR analysis exhibited maximum expression of ToxA and second highest expression of PKS1 genes in brownish black type isolates suggesting most virulent type of isolates among the other morphological isolates. Relative expression of PKS1 showed highest level in black isolates and SCD1 showed second highest although highest level was recorded in greenish black type isolates. All the three target genes showed lowest expression in dull white type and considered as least virulent. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810142819 | |
| dc.keywords | Agriculture, Plant breeding, Wheat, Triticum aestivum (L.), Bipolaris sorokiniana, Genetics, PKS1, SCD1 | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 47p. ; x(Bibliography) | en_US |
| dc.publisher | Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Expression analysis for virulence causing gene of Bipolaris sorokiniana | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Expression analysis for virulence causing gene of Bipolaris sorokiniana | en_US |
| dc.type | Thesis | en_US |