ANTIGENIC CHARACTERIZATION OF EGG DROP SYNDROME-1976 (EDS-76) VIRUS
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Date
1994
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AAU, Anand
Abstract
Egg drop syndrome-1976 (EDS-76) virus has gained importance as one of the major causes of drop in egg production. EDS-78 virus has representative strains like 127 and BC14 which are identical serologically. The possibility of appearance of new isolates can not be ruled out by investigating the variations in immunogenic fractions and their interrelationship say provide indications to select a particular strain for vaccine production and to correlate specific antigenic fractions to immunogenicity of the virus isolate. The present work was on establishing antigenic relationship between strain 127 and two local isolates Sb and Kr of BDS-76 virus employing Haemagglutination inhibition (HI), Agar gel inmunodiffusion (AGID), Counter immunoelectrophoresis (CIEP) and Cross immunoeleotrophoresis (CrIEP). No much significant variation could be observed in the HI titres of sera against strain 127 and isolates Sb and Kr. Thus isolates of EDS-76 were indistinguishable based on HI test. Two common sharing lines were observed in strain 127 and Kr isolate, while one common sharing line was observed in Sb isolate with homologous as well as heterologous system in AGID. More number of precipitation lines were detected in ClEP than that in AGID in homologous and heterologous systens. Closed antigenic relationship was observed among strain 127 and isolates Sb and Kr. In standardized CrIEP using homologous and heterologous system of strain 127 and isolates Sb and Kr, four immuno precipitates were observed in each case. No apparent differences could be established among three isolates (strain 127 and isolates Sb and Kr of EDS-76 virus) by HI test, AGID, CIEP and CrIEP.
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VERTINARY MICROBIOLOGY, CHARACTERIZATION