Agrobacterium-mediated transfer of amaranthus seed albumin (AmA1) gene driven by seed specific promoter in indica rice variety (HKR-46) for improving the protein quality
dc.contributor.advisor | Chowdhury, V.K. | |
dc.contributor.author | Katta, Suman | |
dc.date.accessioned | 2016-11-21T14:28:36Z | |
dc.date.available | 2016-11-21T14:28:36Z | |
dc.date.issued | 2007 | |
dc.description.abstract | Experiments were conducted to transfer Amaranthus seed albumin gene, AmA1, driven by GBSS promoter in indica rice variety HKR46 by Agrobacterium-mediated transformation. The japonica rice variety TNG67 was also used as control for transformation. During the present investigation, 21-24 days old calli induced from mature seed scutella and immature embryos on modified MS medium were used. The calli were co-cultivated for 10 minutes in liquid co-cultivation medium with Agrobacterium strain EHA105 (pSB8G) and then transferred onto the solidified co-cultivation medium for 2-3 days. Then the calli were screened on selection medium containing cefotaxime (250 mg/l) and G-418 (50 mg/l) for 2-3 cycles of selection of 15 days each. A total of 23/1822 calli of HKR46 and 242/345 of TNG67 showed sustained proliferation on the selection medium. The putatively transformed calli were then transferred onto regeneration medium. A total of 9 HKR-46 and 89 TNG-67 plants were regenerated from the selected calli by using the MS based regeneration media supplemented with antibiotics. Eighteen of these plants (HKR-46, 4 plants; TNG-67, 14 plants) were tested for desired bands. An amplified product of 1.1 kb fragment was detected in 13 plants (Table 4.4). The study shows an urgent need of developing an efficient protocol for high frequency plant regeneration of transformed indica and japonica rice varieties. | en_US |
dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/86683 | |
dc.language.iso | en | en_US |
dc.publisher | CCSHAU | en_US |
dc.sub | Biotechnology and Molecular Biology | |
dc.subject | Rice, Transformation, AmA1 gene, Seed specific promoter | en_US |
dc.these.type | M.Sc | |
dc.title | Agrobacterium-mediated transfer of amaranthus seed albumin (AmA1) gene driven by seed specific promoter in indica rice variety (HKR-46) for improving the protein quality | en_US |
dc.type | Thesis | en_US |
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