EVALUATION OF SEMEN QUALITY ON LIQUID STORAGE OF SPECIFIC FRACTIONS OF LARGE WHITE YORKSHIRE BOAR SEMEN
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Date
2019-08-09
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
The present research was carried out to assess the quality of Large White
Yorkshire (LWY) boar semen in specific fractions within an ejaculate and to assess
the keeping quality of fractions of LWY boar semen stored in liquid state at 15C.
Two fractions of the semen were taken for the study, initial 10 mL of sperm rich
fraction (SRF) formed F1/Group I, rest of the sperm rich fraction formed F2/group
II and mixture of half of F1 and F2 each formed group III. A total of 30 ejaculates
were used for the study as fractions from four adult healthy LWY boars (16 in Phase
I and 14 in Phase II). In phase I, differences in fresh semen characteristics of F1 and
F2 were evaluated with respect to pH, concentration, seminal plasma protein content
and SDS PAGE profile were analysed. Significant differences were observed
between fractions with respect to pH and sperm concentration. The sperm
progressive motility and total protein content of semen did not differ significantly
between F1 and F2 fraction. An average number of 13.5 ± 0.51 bands were
observed in F1, 12.92 ± 0.44 bands in F2 and 12.78 ± 0.23 bands in the sperm free
fraction. The proteins observed varied in the molecular weight from 18 - 244 kDa,
8 – 242 kDa and 9 – 248 kDa in F1, F2 and sperm free fraction, respectively. In
Phase II of study, semen fractions were analyzed for their keeping quality at 15C
after being extended in BTS as group I, group II and group III. Semen was extended
with BTS to a final concentration of 50 × 106
sperms/mL and gradually cooled to
22°C, held at same temperature for three hours. The semen was subsequently cooled
to 15°C and stored for 72h and evaluated at 4, 24, 48 and 72 h of storage. The pH
of semen was significantly (p < 0.01) higher in group II at all time point intervals.
The progressive motility of semen was significantly (p < 0.05) higher in group I at
24h, 48 h and 72 h of storage. The sperm viability was significantly higher (p <
0.05) in group I at 72 h of storage alone. The sperm abnormality, acrosome integrity
and rapid HOS response was significantly higher in group I at 24 h, 48 h and 72 h
of storage alone. Sperm plasma membrane integrity showed a trend of being higher
in group I at 72 h of liquid storage (p < 0.1). The cholesterol values at 0h and 72h
was significantly higher (p < 0.01) in group I than other groups. The study revealed
that initial 10 mL of SRF had showed better preservability at 15C than rest of SRF.