Prevalence and immunodiagnosis of sarcocystis sp. of public health significance in cattle
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Date
2001
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
A total of 211 samples were examined between July 2000 to June 2001 to know the prevalence of Sarcocystis sp. of which 128 (60.66%) were found positive for Sarcocystis sp. using pepsin digestion method. 116 (54.32%) samples were found positive by rapid isolation of intact micro-Sarcocystis cysts from muscular tissues. Prevalence of Sarcocystis cruzi, Sarcocystis hirsuta and Sarcocystis hominis were found to be 41.70%, 9.47% and 3.79%, respectively. Only micro-Sarcocystis were encountered. Structure of cyst wall was used as a main criteria for differentiation of Sarcocystis sp. Prevalence of Sarcocystis sp. was found to be positively correlated with increase in age. Month-wise occurrence of Sarcocystis sp. revealed prevalence of 75%, 61.11%, 53.84% and 56.25% in months of August, September, December and June, respectively. Incidence of Sarcocystis sp. in male and female animals was found to be statistically nonsignificant; being 57.14% and 61.53%, respectively. Histopathology of affected tissues revealed presence of Sarcocystis sp. No major histopathological changes associated with cysts were observed. Hyperimmune sera was raised in rabbits. Immune response was detected after 21 days of initial injection of Sacocystis zoite antigen by double immune diffusion (DID). Thirteen (26%) out of 50 serum samples reacted positively to double immunodiffusion. Fifteen (30%) out of 50 serum samples reacted positively to countercurrent immune electrophoresis. Twenty three (46%) out of 50 serum samples reacted positively to Dot-ELISA. Results revealed high sensitivity of Dot-ELIS as compared to double immuno diffusion and counter-current immuno-electrophoresis.