Evaluation of mushroom growth promoting microbial isolates for enhancement of yield potential in Calocybe indica
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Date
2023
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Punjab Agricultural University
Abstract
Mushroom growth promotion by means of biological agents is an emerging aspect in mushroom cultivation. The present study was aimed to search for a novel bacterium potentially able to enhance the growth and yield of Calocybe indica, also known as milky mushroom. Soil samples from 6 sites supporting natural growth of macro-fungi were collected and screened for isolates. Out of the total 79 isolates, 5 isolates were selected for further analysis on the basis of mushroom growth promoting traits. During the in vitro radial and linear growth rate evaluation through co-cultivation of isolates with C. indica mycelium, isolate B28 was observed to enhance the mycelial growth (7.10±0.10 mm/day) as well as the linear growth rate (4.94±0.14 mm/day) compared to control (6.47 and 4.30, respectively). Isolate B28 was used at various concentrations during the cultivation of C. indica. It has been observed that the number of days of spawn run were reduced in case of supplementation at the rate of 5 ml/bag (20±1). Percentage increase biological efficiency (20.63%) of the crop was also observed in case of 5ml supplementation of isolate in comparison to control. At higher
concentrations (25 ml/bag), biological efficiency was found to be decreased by even as much as 86.78%. It can be concluded that the use of isolated bacteria (B28) at an optimal concentration (3ml and 5 ml/bag of 108 CFU/ml culture) in both the substrate and casing layer can decrease the number of days of spawn run, number of days for pinning, and can increase the number of fruiting bodies, and yield of the crop, hence resulting in increase in biological efficiency.
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Navneet Kaur (2023). Evaluation of mushroom growth promoting microbial isolates for enhancement of yield potential in Calocybe indica (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.