CRYOPROTECTIVE EFFECT OF BUCK SEMEN SUPPLEMENTED WITH DIMETHYLACETAMIDE AND L-GLUTAMINE IN A TRIS BASED EXTENDER
| dc.contributor.advisor | Dr. Leeba Chacko | |
| dc.contributor.author | KANIMOZHI T.M | |
| dc.date.accessioned | 2024-01-24T12:34:39Z | |
| dc.date.available | 2024-01-24T12:34:39Z | |
| dc.date.issued | 2022-12-23 | |
| dc.description.abstract | A study was designed with the objective to assess the cryoprotective effect of dimethylacetamide (DMA) addition to Tris based buck semen extender and to evaluate the synergistic effect of DMA and L-glutamine on post-thaw semen quality. Ten ejaculates were taken from four Malabari bucks. After preliminary evaluation, pooled samples was extended in four different extender including control, 3 per cent DMA, control with L-glutamine and DMA with L-glutamine. Pre-freeze and post-thaw evaluation including sperm kinectics was studied. Post thaw motility, viability, HOST and acrosome integrity (56.26 ± 3.54; 48.25 ± 0.75; 59.5±1.13; 59.88 ± 2.51 per cent), respectively was significantly higher (p<0.05) and low MDA values (2.22 ± 0.15 μmol/mL) in control with L-glutamine group was noticed whereas DMA group had lower post-thaw motility, viability, HOST and acrosome integrity (32.19 ± 3.28; 35.13 ± 1.17; 41.25 ± 1.52; 43.00 ± 1.17), respectively and high MDA value (4.11 ± 0.25 μmol/mL). From the present study it was concluded Lglutamine had synergistic effect with glycerol and manual freezing of Malabari buck sperm with DMA was not feasible comparing to glycerol. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810206541 | |
| dc.keywords | CRYOPROTECTIVE EFFECT OF BUCK SEMEN SUPPLEMENTED WITH DIMETHYLACETAMIDE AND L-GLUTAMINE IN A TRIS BASED EXTENDER | |
| dc.keywords | CRYOPROTECTIVE EFFECT | |
| dc.language.iso | English | |
| dc.pages | 115 | |
| dc.publisher | COLLEGE OF VETERINARY AND ANIMAL SCIENCS, POOKODE, WAYANAD, KERALA VETERINARY AND ANIMAL SCIENCES UNIVERSITY | |
| dc.research.problem | Assessment of the cryoprotective effect of dimethylacetamide (DMA) addition to tris based buck semen extender , Study of the synergistic effect of DMA and L-glutamine on post thaw semen quality | |
| dc.sub | Animal Reproduction, Gynaecology and Obstetrics | |
| dc.theme | Cryoprotective effect of buck semen supplemented with dimethylacetamide and L glutamine in a TRIS based extender | |
| dc.these.type | M.V.Sc. | |
| dc.title | CRYOPROTECTIVE EFFECT OF BUCK SEMEN SUPPLEMENTED WITH DIMETHYLACETAMIDE AND L-GLUTAMINE IN A TRIS BASED EXTENDER | |
| dc.type | Thesis |