Effect of glutathione, proline and magnesium sulphate on seed vigour and antioxidants in sorghum (Sorghum bicolor [L.] Moench) under salt stress conditions
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Date
2024
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Punjab Agricultural University
Abstract
In the present investigation, effect of glutathione, proline and magnesium sulphate was studied on seed vigour and antioxidant defense system under salt stress conditions in sorghum (Sorghum bicolor [L.] Moench) cvs. SL 45 and SL 46. The salt stress had adverse effects on seed germination and seed vigour parameters. Seeds of sorghum were treated with proline (25ppm, 50 ppm and 100 ppm), glutathione (100 ppm, 250 ppm and 500 ppm) and magnesium sulphate (10 ppm, 25 ppm and 50 ppm) and were subjected to salt stress of 50 mM and 75 mM. Different parameters were recorded from seedlings after 10 days of germination. The seed treatments increased the physiological (percent germination, seedling length, seedling biomass, vigour index I and II) and biochemical parameters (total soluble sugars, total soluble proteins, proline content and activities of antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase) of seedlings as compared to control. However, malondialdehyde content decreased with seed treatments at all salinity levels in both the cultivars. The results showed that proline 100 ppm, glutathione 500 ppm, magnesium sulphate 50 ppm were more effective than proline (25 ppm, 50 ppm), glutathione (100 ppm, 250 ppm), magnesium sulphate (10 ppm, 25 ppm) respectively. Thus, the application of proline, glutathione and magnesium sulphate could play an important role in improving the salt stress tolerance by their osmoprotective role.
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Maheshwari, Akshita (2024). Effect of glutathione, proline and magnesium sulphate on seed vigour and antioxidants in sorghum (Sorghum bicolor [L.] Moench) under salt stress conditions (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.