Karyological studies vis-à-vis wool quality traits in Nali, Munjal and Crossbred sheep

dc.contributor.advisorSikka, A.K.
dc.contributor.authorChaudhary, Pradeep
dc.date.accessioned2017-08-01T10:11:03Z
dc.date.available2017-08-01T10:11:03Z
dc.date.issued2012
dc.description.abstractKaryological studies were made on somatic chromosomes in Nali, Munjal and Crossbred sheep. A total number of 50 animals screened for cytogenetic studies and 115 animals for analysing wool quality traits. In vitro whole-blood-culture technique was employed for the preparation of somatic metaphase chromosomes. Relative lengths of autosomes and sex chromosomes were compared in three groups. Morphometric measurements like arm ratio and centromeric index were also analysed comparatively. Model karyotypes and ideograms were constructed on the basis of morphological homology and bandings (G-and Ag NORs). Somatic metaphase chromosomes in all the three groups reaffirms diploid count of fifty- four (2N=54). Among the 26 pairs of autosomes, first 3 pairs of chromosomes were metacentric/sub metacentric type. Rest 23 pairs of autosomes were acrocentric type. The similar pattern of chromosomes between the Nali Munjal and Crossbred sheep was observed for the shape of autosomes and sex chromosomes. The chromosomes (autosomes and sex chromosomes) were linearly differentiated for morphometric analysis into three groups on the basis of size and shape. Group ‘A’ consisted of 3 pairs of large sub metacentric autosomes, and group ‘B’ consisted of rest (4 to 26 pairs) of acrocentric autosomes. The ‘C’ group comprised of the sex chromosomes X and Y. The centromeric indices for the first 3 pairs of autosomes showed small but non-significant differences among Nali, Munjal and Crossbred sheep. Among the three chromosome pairs the centromeric indices showed descending trend with the lowest value for third chromosome pair. However, the arm ratio showed reverse trend with lowest value for the third pair and highest value for the first pair with an indication of the chromosomes to be exhibiting metacentric type of shape from its sub metacentric type. G-banding pattern of chromosomes was similar in Nali, Munjal and Crossbred sheep. Ag-NOR banding revealed five pairs of chromosomes possessing NOR sites in all groups. High resolution banding revelled presence of lesser number of bands in Crossbred sheep as compared to indigenous sheep breeds. Wool quality traits study lead to conclusion that Nali and Munjal differ significantly from Crossbred sheep in terms of their wool quality parameters. These three breeds shared no specific chromosomal landmarks for their differences in wool texture.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810026992
dc.keywordsKaryotypes, Relative length, Arm ratio, Centromeric index, G-Band and NOR bands, High resolution banding, Wool quality traits, Nali, Munjal, Crossbred sheep.en_US
dc.language.isoenen_US
dc.publisherLUVASen_US
dc.subAnimal Genetics and Breedingen_US
dc.these.typeM.V.Sc.
dc.titleKaryological studies vis-à-vis wool quality traits in Nali, Munjal and Crossbred sheepen_US
dc.typeThesisen_US
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