Morphological characterization and assessment of genetic diversity in interspecific derivatives of chickpea (Cicer arietinum L.)
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Date
2022
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Punjab Agricultural University
Abstract
In the present investigation, a set of 90 interspecific derivatives, derived from four different crosses between cultivated chickpea (PBG5 and BGD72) and wild species (Cicer reticulatum ILWC229 and C. echinospermum ILWC229) were evaluated along with their parents and two standard checks (PBG7, PBG8) to determine the variability, association, divergence and resistance to Ascochyta Blight and Botrytis Grey Mould diseases during 2020-21 and 2021-22 in the experimental area of pulses section, PAU, Ludhiana. The genotypes were planted in αlattice design with two replications. ANOVA revealed significant differences among the derivatives for all the traits studied. Phenotypic and genotypic coefficients of variation were high for the number of pods per plant followed by primary and secondary branches per plant and 100-seed weight. High heritability estimates were observed for plant height followed by days to flowering, harvest index, days to maturity, number of pods per plant and biological yield per plot. Seed yield per plot had a positive and highly significant correlation with biological yield per plot, plant height, harvest index, 100-seed weight and the number of pods per plant. Path analysis revealed highest positive direct effect of biological yield per plot on seed yield per plot, followed by harvest index, secondary branches per plant, days to 50% flowering, 100-seed weight and days to maturity. Based on genetic diversity analysis, 90 derivatives were grouped into 5 clusters. The maximum inter-cluster distance was observed between clusters II and V. Cluster II was good with respect to seed yield and biological yield per plot, 100-seed weight and days to 50% flowering and maturity. A total of 33 derivatives were found to be resistant to AB disease and 12 were found to be moderately resistant to BGM disease. The parental lines were screened for polymorphism using 450 SSR markers. The introgression from C. reticulatum and C. echinospermum was confirmed by graphical genotyping. Resistance to AB was confirmed by markers viz. TR19, TA194, TA96, TA106 and H1I16 and resistance to BGM by TA194, TA140 and TA118. In addition, TA113 and TA18 were related to higher seed weight and tallness, respectively. Therefore, these markers can be used with sufficient confidence in marker-assisted selection to develop superior lines for resistance to AB and BGM along with higher seed weight and plant height.
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Vadithya, Amool Singh (2022). Morphological characterization and assessment of genetic diversity in interspecific derivatives of chickpea (Cicer arietinum L.) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.