STUDIES ON PURPLE BLOTCH [Alternaria porri (Ellis) Cif.] OF ONION [Allium cepa L.] AND ITS MANAGEMENT

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dc.contributor.advisorDr. S. T. Patel
dc.contributor.authorPANKAJ KUMAR SINGH
dc.date.accessioned2017-04-11T12:01:50Z
dc.date.available2017-04-11T12:01:50Z
dc.date.issued2012
dc.description.abstractPurple blotch of onion caused by Alternaria porri (Ellis) Cif. is one of the important foliar diseases. Present investigation was carried out at AAU Anand during the year 2010-12 on cultural and morphological characterization of the pathogen, protein profiling of the six isolates of A. porri by SDS PAGE, biological interaction of phylloplane mycofloras of onion against A. porri, in vitro efficacy of fungicides and bioagents on the growth of pathogen, and evaluation of fungicides and bioagents against purple blotch of onion under field conditions. The pathogen was isolated from infected onion leaves collected from six different onion growing areas of Gujarat. The isolates were named as AP 1, AP 2, AP 3, AP 4, AP 5 and AP 6 for Anand, Kheda, Bhavnagar, Amreli, Junagadh and Rajkot districts, respectively. Pathogenicity test of each of the six isolates was proved by inoculating the spore suspension and homogenized mycelial bits (1× 104 conidia/ml) of A. porri on healthy onion seedlings. Pathogen was identified as Alternaria porri. Protein profile by SDS-PAGE of six isolates of A. porri showed variability in terms of number of protein bands. On the basis of dendrogram, first main cluster consisted of isolates AP 1, AP 2, AP 3, AP 4 and AP 5, whereas the second cluster included only AP 6 isolate. Evaluation of eight phylloplane mycofloras against A. porri under in vitro revealed relatively higher antagonistic capability of Aspergillus niger, Trichoderma sp., Aspergillus flavus and Aurobasidium sp. as compared to Alternaria alternata, Curvularia sp., Cladosporium sp. and Fusarium sp. Among the nine fungicides tested in vitro for their efficacy, Azoxystrobin and Propiconazole completely inhibited the mycelial growth of A. porri at all the three concentrations. The next effective fungicides were Carbendazim 12% + Mancozeb 63%, Propineb and Hexaconazole at 0.15, 0.2 and 0.025 per cent, respectively. Trichoderma viride, T. harzianum and T. virens were found more effective as compared to Pseudomonas fluorescens and Bacillus subtilis under in vitro evaluation against A. porri. Under field conditions, purple blotch of onion can be effectively managed by three sprays of Propiconazole @ 0.01 per cent followed by three sprays of Mancozeb @ 0.15 per cent and Azoxystrobin @ 0.025 per cent. Trichoderma sp. showed higher antagonistic capability under in vitro, but failed to perform in vivo.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810007151
dc.keywordspurple blotch, onionen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemSTUDIES ON PURPLE BLOTCH [Alternaria porri (Ellis) Cif.] OF ONION [Allium cepa L.] AND ITS MANAGEMENTen_US
dc.subPlant Pathologyen_US
dc.subjectagriculture, plant pathologyen_US
dc.subjectstudyen_US
dc.themePURPLE BLOTCH [Alternaria porri (Ellis) Cif.]en_US
dc.these.typeM.Scen_US
dc.titleSTUDIES ON PURPLE BLOTCH [Alternaria porri (Ellis) Cif.] OF ONION [Allium cepa L.] AND ITS MANAGEMENTen_US
dc.typeThesisen_US
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