STUDIES ON PURPLE BLOTCH [Alternaria porri (Ellis) Cif.] OF ONION [Allium cepa L.] AND ITS MANAGEMENT
dc.contributor.advisor | Dr. S. T. Patel | |
dc.contributor.author | PANKAJ KUMAR SINGH | |
dc.date.accessioned | 2017-04-11T12:01:50Z | |
dc.date.available | 2017-04-11T12:01:50Z | |
dc.date.issued | 2012 | |
dc.description.abstract | Purple blotch of onion caused by Alternaria porri (Ellis) Cif. is one of the important foliar diseases. Present investigation was carried out at AAU Anand during the year 2010-12 on cultural and morphological characterization of the pathogen, protein profiling of the six isolates of A. porri by SDS PAGE, biological interaction of phylloplane mycofloras of onion against A. porri, in vitro efficacy of fungicides and bioagents on the growth of pathogen, and evaluation of fungicides and bioagents against purple blotch of onion under field conditions. The pathogen was isolated from infected onion leaves collected from six different onion growing areas of Gujarat. The isolates were named as AP 1, AP 2, AP 3, AP 4, AP 5 and AP 6 for Anand, Kheda, Bhavnagar, Amreli, Junagadh and Rajkot districts, respectively. Pathogenicity test of each of the six isolates was proved by inoculating the spore suspension and homogenized mycelial bits (1× 104 conidia/ml) of A. porri on healthy onion seedlings. Pathogen was identified as Alternaria porri. Protein profile by SDS-PAGE of six isolates of A. porri showed variability in terms of number of protein bands. On the basis of dendrogram, first main cluster consisted of isolates AP 1, AP 2, AP 3, AP 4 and AP 5, whereas the second cluster included only AP 6 isolate. Evaluation of eight phylloplane mycofloras against A. porri under in vitro revealed relatively higher antagonistic capability of Aspergillus niger, Trichoderma sp., Aspergillus flavus and Aurobasidium sp. as compared to Alternaria alternata, Curvularia sp., Cladosporium sp. and Fusarium sp. Among the nine fungicides tested in vitro for their efficacy, Azoxystrobin and Propiconazole completely inhibited the mycelial growth of A. porri at all the three concentrations. The next effective fungicides were Carbendazim 12% + Mancozeb 63%, Propineb and Hexaconazole at 0.15, 0.2 and 0.025 per cent, respectively. Trichoderma viride, T. harzianum and T. virens were found more effective as compared to Pseudomonas fluorescens and Bacillus subtilis under in vitro evaluation against A. porri. Under field conditions, purple blotch of onion can be effectively managed by three sprays of Propiconazole @ 0.01 per cent followed by three sprays of Mancozeb @ 0.15 per cent and Azoxystrobin @ 0.025 per cent. Trichoderma sp. showed higher antagonistic capability under in vitro, but failed to perform in vivo. | en_US |
dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810007151 | |
dc.keywords | purple blotch, onion | en_US |
dc.language.iso | en | en_US |
dc.publisher | AAU, Anand | en_US |
dc.research.problem | STUDIES ON PURPLE BLOTCH [Alternaria porri (Ellis) Cif.] OF ONION [Allium cepa L.] AND ITS MANAGEMENT | en_US |
dc.sub | Plant Pathology | en_US |
dc.subject | agriculture, plant pathology | en_US |
dc.subject | study | en_US |
dc.theme | PURPLE BLOTCH [Alternaria porri (Ellis) Cif.] | en_US |
dc.these.type | M.Sc | en_US |
dc.title | STUDIES ON PURPLE BLOTCH [Alternaria porri (Ellis) Cif.] OF ONION [Allium cepa L.] AND ITS MANAGEMENT | en_US |
dc.type | Thesis | en_US |
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