HEPATOPROTECTIVE EFFECT OF ECLIPTA PROSTRATA (L.) L. LEAVES ON EXPERIMENTALLY INDUCED AFLATOXICOSIS IN BROILER CHICKEN

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dc.contributor.advisorPreethy John
dc.contributor.authorPRIYA. K
dc.date.accessioned2023-01-06T10:04:20Z
dc.date.available2023-01-06T10:04:20Z
dc.date.issued2018-11-30
dc.descriptionThesis Submitted in partial fulfilment of the requirement for the degree of Master of Veterinary Science In Veterinary Pharmacology and Toxicologyen_US
dc.description.abstractThe study was aimed to investigate the hepatoprotective effect of E. prostrata (Kayyonni) leaf powder on experimentally induced aflatoxicosis in broiler chicken. The leaf powder was subjected to preliminary phytochemical screening to find out the active principles present in it. Aflatoxin was produced in maize using the culture Aspergillus flavus NRRL 6513. The maize culture powder yielded 143.48 ppm of aflatoxin. This mouldy maize was incorporated in experimental feed to arrive 500 ppb of aflatoxin. Sixty Cobb400 day old broiler chicks weighing 50 ± 5 g were randomly divided into six groups comprising 10 birds in each group. The birds were maintained under deep litter system and provided with ad libitum water and feed throughout the experimental period. All the birds were vaccinated as per the standard schedule. Aflatoxicosis was experimentally induced in all groups except T1 and T3 by giving 500 ppb of aflatoxin B1 (AFB1) from eighth day of age onwards. The group T1 was kept as normal control and T2 as toxic control. T3 was fed with E. prostata leaf powder at 0.2 per cent level. The leaf powder of E. prostrata was given to T4, T5 and T6 at dose rates of 0.05, 0.1 and 0.2 per cent respectivelyBody weight was recorded at weekly intervals and the blood was collected from the wing vein on days 7, 21 and 42. Serum was separated and used for the estimation of biochemical parameters such as aspartate transaminase (AST), creatine kinase (CK), cholesterol and total proteins. On the day 42, all the birds were sacrificed; detailed post- mortem examination was conducted. Liver samples were taken to estimate antioxidant parameters such as lipid peroxidation (LPO) and reduced glutathione (GSH). Representative liver samples were also taken and preserved with 10 per cent neutral buffered formalin for histopathological examinationThe preliminary phytochemical screening of E. prostrata leaf powder revealed the presence of steroid, tannins, flavonoids, diterpenes, tripterpenes and saponin.Treatment with E. prostrata leaves powder revealed hepatoprotection in dose dependent manner which is indicated by significant (P<0.05) reduction in the level of serum AST and increase in the level of cholesterol and total protein. The oxidative stress induced by aflatoxin in liver was reduced to a great extend as indicated by the increased level of reduced glutathione and decrease in the lipid peroxidation. Histopathological examination of liver showed regenerative changes in a dose dependent manner when compared with that of normal control group. Thus, it could be concluded that E .prostrata leaf powder had marked antioxidant and hepatoprotective effect on experimentally induced aflatoxicosis in broiler chickenen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810191375
dc.keywordsHEPATOPROTECTIVE EFFECT, ECLIPTA PROSTRATA (L.) L., LEAVES, EXPERIMENTALLY INDUCED AFLATOXICOSIS, BROILER CHICKEN, Eclipta prostrate, Hepatoprotective effect of E. prostrata, Aflatoxicosis in broiler chicken Serum biochemical parameters, Gross pathological lesions , Histopathology of liveren_US
dc.language.isoEnglishen_US
dc.pages123p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHYen_US
dc.research.problemTo evaluate the hepatoprotective effect of leaf powder of Eclipta prostrata (L.) L. (Kayyonni) on experimentally induced aflatoxicosis in broiler chicken.en_US
dc.subVeterinary Pharmacology and Toxicologyen_US
dc.themeHEPATOPROTECTIVE EFFECT OF ECLIPTA PROSTRATA (L.) L. LEAVES ON EXPERIMENTALLY INDUCED AFLATOXICOSIS IN BROILER CHICKENen_US
dc.these.typeM.V.Sc.en_US
dc.titleHEPATOPROTECTIVE EFFECT OF ECLIPTA PROSTRATA (L.) L. LEAVES ON EXPERIMENTALLY INDUCED AFLATOXICOSIS IN BROILER CHICKENen_US
dc.typeThesisen_US
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