MANAGEMENT OF ROOT KNOT NEMATODE (Meloidogyne incognita) IN TOMATO BY BACTERIAL BIOAGENT
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Date
2019-09
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AAU, Jorhat
Abstract
Investigations were carried out to evaluate the efficacy of bacterial bioagents viz., Bacillus subtilis, Bacillus megaterium, Bacillus pumilus and Pseudomonas fluorescens as seed as well as soil treatment against root knot nematode, Meloidogyne incognita on tomato (var. Pusa Ruby). Seed treatment experiment was conducted with thirteen treatments viz., T1: B. subtilis (1×109cfu/gm of talc formulation) @ 10gm/kg of seed, T2: B. subtilis (1×109cfu/gm of talc formulation) @ 20gm/kg of seed; T3: B. subtilis (1×109 cfu/gm of vermi formulation) @ 10 gm/kg of seed; T4: B. subtilis (1×109 cfu/gm of vermi formulation @ 20 gm/kg of seed; T5: B. pumilus (1×109cfu/gm of talc formulation) @ 10gm/kg of seed; T6: B. pumilus (1×109cfu/gm of talc formulation) @ 20gm/kg of seed; T7: B. megaterium (1×109cfu/gm of talc formulation) @ 10gm/kg of seed; T8: B. megaterium (1×109cfu/gm of talc formulation) @ 20gm/kg of seed; T9: B. megaterium (1×109 cfu/gm of vermi formulation) @10gm/kg of seed; T10: B. megaterium (1×109 cfu/gm of vermi formulation) @ 20gm/kg of seed; T11: Pseudomonas fluorescens (1×109cfu/gm of talc formulation) @ 10gm/kg of seed; T12: P. fluorescense (1×109cfu/gm of talc formulation) @20/kg of seed and T13: untreated control. Results revealed that there were a significant increase in plant growth parameters and significant decrease in galls, eggmass and soil nematode population in all treatments over untreated control. However, seed treated with Pseudomonas fluorescens @ 20g/kg seed recorded the best result in increasing the plant growth parameters of tomato and reducing the root knot nematode multiplication followed by Bacillus subtilis (vermi formulation) @ 20g/kg seed. Pot experiment was conducted to evaluate the efficacy of bacterial bioagents as soil application with thirteen treatments viz., T1: Bacillus subtilis (1×109cfu/gm of talc formulation) @ 2%(w/w); T2: B. subtilis (1×109cfu/gm of talc formulation) @ 3%(w/w); T3: B. subtilis (1×109 cfu/gm of vermi formulation) @ 2%(w/w); T4: B. subtilis (1×109 cfu/gm of vermi formulation @ 3% (w/w); T5: B. pumilus (1×109cfu/gm of talc formulation) @ 2%(w/w); T6: B. pumilus (1×109cfu/gm of talc formulation) @ 2% (w/w); T7: B. megaterium (1×109cfu/gm of talc formulation) @ 3% (w/w); T8: B. megaterium (1×109cfu/gm of talc formulation) @ 2% (w/w); T9: B. megaterium (1×109 cfu/gm of vermi formulation) @ 3% (w/w); T10: B. megaterium
(1×109 cfu/gm of vermin formulation) @ 3% (w/w); T11: Pseudomonas fluorescens (1×109cfu/gm of talc formulation) @ 2% (w/w); T12: Pseudomonas fluorescens (1×109cfu/gm of talc formulation) @3% (w/w) and T13: Untreated control; The result revealed that all the treatments significantly increased plant growth parameters and significantly decreased gall per root system, eggmass per root system and final root knot nematode population in soil over untreated control. However, maximum plant growth parameters and reduction in root knot nematode multiplication were recorded in soil treated with Pseudomonas fluorescens @ 3% (w/w) followed by Bacillus subtilis (vermi formulation) @ 3% (w/w) whereas minimum was recorded in untreated control
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