Runner production of strawberry and its survivability in subtropical condition of Eastern India
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Date
2019-10-04
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Department of Horticulture (Fruit & Fruit Technology), BAU, Sabour
Abstract
The cultivated strawberry (Fragaria x ananassa Duch.) belongs to family Rosaceae is a natural hybrid, derived from two North American species, Scarlet (Fragaria virginiana Duch.) and Chilean (Fragaria chiloensis Duch.). This is an octaploid (2n=8x=56) developed in France during the 17th Century. Due to popularity of strawberry, there is need of cultivation of strawberry but insufficient planting material is major limiting factor. As we know, commercially strawberry is propagated by runner therefore keeping the facts we need huge planting materials for the fulfilment of cultivation of strawberry. In view of potential commercial value of this crop, it is highly desirable to develop an efficient tissue culture protocol as well as runner production in field condition for rapid and large scale multiplication of strawberry under Bihar conditions.
In the experimental results, sterilizing agent HgCl2 @ 0.1 % for 3 minutes was effective for 70 % establishment. The other parameters like the minimum number of days taken for shoot proliferation (21.67) and the maximum number of shoot generated per explant (5.40) were found in MS media supplemented with BAP @ 3.0 mg/l + NAA @ 0.5 mg/l respectively. The maximum shoot length (5.80 cm) was found in MS media consisting with BAP @ 3.0 mg/l and the maximum number of leaves per generated shoots (6.04) was found in MS media added with BAP @ 4.5 mg/l + NAA @ 0.5 mg/l. The maximum root formation (53.96 %), root length (4.80 cm), number of root generated per shoot (6.80) and the number of days taken for root initiation (18.40) were achieved in half MS media consisting with IAA @ 1.5 mg/l.
Strawberry plantlets obtained through micropropagation exhibited 20-60 per cent survival during acclimatization in different potting mixtures. In the field condition, the maximum plant height (28.54 cm), plant spread (39.00 cm), crown diameter (42.68 mm), petiole length (12.53 cm), number of leaves per plant (25.60), number of runner per plant (15.73), runner length (23.27 cm) and leaf area (36.52 cm2) were found with application of GA3 @ 250 ppm.
The results described various factors that influence the phenolic exudation, establishment, shoot proliferation, shoot multiplication, root induction and acclimatization of strawberry as well as runner production with application of GA3 @ 250 ppm in order to make it commercial production of strawberry planting materials for future perspective.