Collection cataloguing and evaluation of rouwolfia spp.
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Date
1993
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Department of Agricultural Botany, College of Horticulture,Vellanikkara
Abstract
A study on 'Collection, cataloguing and evaluation of Rauwolfia spp' was conducted in the Department of Agricultural Botany, College of Horticulture, Vellanikkara during 1991-93, with the objectives of understanding the distribution pattern of various species of Rauwolfia in Kerala, detailed descriptive study of the morphological and anatomical characters of the different species of Rauwolfia and a preliminary comparative evaluation study for the total alkaloids in roots and the chlorophyll content of aerial parts.
Survey of 10 geographic locations of Kerala from North to South was conducted and four species of Rauwolfia were collected. Nine ecotypes of R. Serpentina and four ecotypes of R. tetraphylla were also collected.
Different species were compared based on 60 morphological characters, 15 anatomical characters and three characters of pollen grains. Evaluation for total root alkaloids was done using chloroform as solvent and determination of total chlorophyll content was done using acetone as solvent.
The study on distribution aspects showed that R. serpentina was widely distributed in Kerala but the frequency of occurrence was low, while R. tetraphylla was widely distributed in non-forest areas only, with a higher frequency of occurence. R. densiflora and R. beddomei are in a state of near extinction while R. micrantha was almost disappeared from Kerala.
Morphological and antomical characters and the morphology and viability of pollen grains showed wide variability among different species of Rauwolfia. Characters in addition to that available in the literature, for identifying the four species of Rauwolfia are suggeated. It is seen that chances are there for the occurence of higher ploids of the same species having higher alkaloid content, in Rauwolfia.
Total alkaloid content of roots, chloroform extract and total chlorophyll content of aerial parts varied with different species and ecotypes of Rauwolfia. The conditions for the higher root alkaloid production in R. sepentina may not be favourable for the alkaloid production in R. tetraphylla.
The chloroform extract and total chlorophyll content of aerial parts were negatively correlated to the total root alkaloid content in all the species of Rauwolfia.
The relationship between these was found to be
“Total root alkaloid content= 2.047 – 0.016 x chloroform extract of aerial parts
=2.304–1.434 x total chlorophyll
content of aerial parts”
The relationship can be effectively utilized in the estimation of root alkaloid, in Rauwolfia spp., even at the early stages of growth, without uprooting the plants.
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170462