ISOLATION AND CHARACTERIZATION OF Riemerella anatipestifer FROM DUCKS
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Date
2011
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
Six bacterial isolates from ducks were characterized as Riemerella
anatipestifer using morphological, cultural and biochemical characters. Duck
isolate of Pasteurella multocida serotype A was used for comparison. All the R.
anatipestifer isolates showed similar biochemical characters except for urease
reaction and sugar fermentation. Riemerella anatipestifer and P. multocida were
differentiated using tests like indole production, gelatin liquefaction, ornithine
decarboxylase utilization and fermentation of glucose. All the R. anatipestifer
isolates were sensitive to chloramphenicol, ciprofloxacin, enrofloxacin,
norfloxacin, gentamicin, clindamycin, doxycycline and cefuroxime. The R.
anatipestifer isolates were found to be non-pathogenic to mice, but highly
pathogenic to ducklings when inoculated subcutaneously and intramuscularly.
The whole cell protein profiles of all the R. anatipestifer isolates were
similar, which was different from that of P. multocida. Six protein bands of
molecular weights 93, 70, 60, 52, 40 and 28 kDa were intensely stained in R.
anatipestifer profile.
Restriction endonuclease analysis of genomic DNA was carried out using
Hinf I, EcoR I, Not I and Hha I. Hinf I revealed polymorphic profiles, EcoR I
yielded indistinguishable smearing pattern, Hha I showed similar pattern for all
the isolates and Not I revealed no digestion. The Hinf I was more discriminatory
as it could group all the isolates in to two profiles. Pasteurella multocida showed
a different restriction enzyme pattern for Hinf I and Hha I.
The different molecular techniques used in this study showed homogeneity
in protein profile and genetic heterogeneity among field isolates of R.
anatipestifer. Among the restriction enzymes used in the DNA fingerprinting of
R. anatipestifer by restriction enzyme analysis, Hinf I was found to have more
discriminatory power
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