Evaluation of trace elements and heavy metal analysis in commercial tea
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Date
2022
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Punjab Agricultural University, Ludhiana
Abstract
Tea is one of the world's most popular non-alcoholic beverage owing to its nutritional value and antioxidant potential due to catechins and flavonoids. Recently, the consumption rate of various teas has increased rapidly. Stringent guidelines laid by food regulations by FSSAI, EU, USFDA etc. for tea have made the analysis of inorganic metal contaminants as a mandatory requirement for its sa consumption as well as export. ICP-MS (inductively coupled plasma mass spectrometry) is among the most sensitive equipment for analysis of the same at trace and ultra-trace levels but with the use of validated method. The study was
therefore planned to validate ICP-MS method for analysis of heavy metals and trace elements in three types of tea viz. green, black and herbal along with their nutritional status. High sensitivity was observed with low LOD and LOQ values for both heavy metals (Chromium, Arsenic, Cadmium, Nickel, Mercury, Lead) and trace metals (Copper, Zinc, Selenium, Cobalt) in various teas in the range of 0.003 to 0.620 µg/kg and 0.010 to 2.048 µg/kg respectively. The linearity for all metals was obtained with R≥ 0.995 and accuracy of the method was established by spiked experiments where recoveries were achieved in the range of 75 to 125%. Precision data indicated very small %RSD ranging 0.46% to 13.44% For nutritional analysis, total polyphenol content (TPC) and antioxidant capacity of three tea, was carried out in relation to steep time where 15 min steep time was optimized for maximum extraction of the both. The study indicated good correlation among the both parameters.
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Manbeer Singh (2022). Evaluation of trace elements and heavy metal analysis in commercial tea (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.