BIOCHEMICAL TECHNIQUES FOR RICE (Oryza sativa L.) VARIETY IDENTIFICATION

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dc.contributor.advisorTalati, J. G.
dc.contributor.authorSINGH, DIWAKAR
dc.date.accessioned2018-06-19T10:12:11Z
dc.date.available2018-06-19T10:12:11Z
dc.date.issued2004
dc.description.abstractInvestigations were carried out at the Departments of Biochemistry, Agriculture Botany & Biotechnology, B.A. College of Agriculture, A.A.U., Anand and Department of Animal Genetics & Breeding, College of Veterinary Sciences, A.A.U., Anand, to study and compare biochemical techniques along with traditional techniques for Rice (Oryza sativa L.) variety identification. Study on morphological and chemical tests revealed that it could be useful for grouping of rice varieties/genotypes, while, modified phenol test was useful to identify all fourteen rice varieties/genotypes. Proximate analysis was carried out to study the nutritional composition of milled rice. Nutritional composition showed little variation and thus, was not useful for varietal identification. Peroxidase, polyphenol oxidase and ∝-amylase enzyme activity showed significant differences and could be applied for varietal identification at 5 & 10 days after germination (DAG), 10 DAG and 20 & 30 DAG respectively, while, acid phosphatase and esterase enzyme activity could not provide useful information regarding varietal identification and thus, was not found useful. Electrophoresis of peroxidase (5 & 10 DAG) and esterase (5, 10 & 20 DAG) isozymes was useful for identification of varieties as well as identification of parents and hybrid and may serve as marker isozyme for rice varieties. Similarly Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of seed storage protein and leaf protein (5 DAG) showed variability and could be effectively useiffor identification of rice varieties/genotypes on the basis of variations in banding pattem and intensity of bands. Randomly amplified polymorphic DNA (RAPD) produced total 180 useful markers of which 77% were polymorphic. All fourteen varieties/genotypes were classified into three different clusters, where variety P-203 was most diverge one. Varieties could be identified on the basis of specific banding pattern. Parents and hybrid also produced differences in banding pattern and could be identified. IR 58025 A line could also be differentiated from its B line on the basis of differences in banding pattern. All the tests conclusively proved that RAPD, SDS-PAGE and isozyme electrophoresis offer a potentially quick, simple, rapid and reliable method for rice varieties/genotypes identification and recognition of lines. Modified phenol test also proved to be rapid and cheap laboratory chemical test for identification of rice varieties, whereas, other chemical and morphological tests can supplement for varietal identification. RAPD and SDS-PAGE were more useful than isozyme electrophoresis because potential number of loci warn more compared to isozymes.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810054280
dc.keywordsBIOCHEMICAL TECHNIQUES, RICE (Oryza sativa L.), VARIETY IDENTIFICATIONen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemBIOCHEMICAL TECHNIQUES FOR RICE (Oryza sativa L.) VARIETY IDENTIFICATIONen_US
dc.subBiochemistryen_US
dc.subjectBIOCHEMISTRY, AGRICULTUREen_US
dc.subjectTECHNIQUEen_US
dc.themeBIOCHEMICAL TECHNIQUES FOR RICE (Oryza sativa L.) VARIETY IDENTIFICATIONen_US
dc.these.typeM.Scen_US
dc.titleBIOCHEMICAL TECHNIQUES FOR RICE (Oryza sativa L.) VARIETY IDENTIFICATIONen_US
dc.typeThesisen_US
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