Calocybe indica strain evaluation for lignocellulolytic enzymes in relation to mushroom yield
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Date
2019
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Punjab Agricultural University, Ludhiana
Abstract
Calocybe indica, milky mushroom is a tropical mushroom of Indian origin that grows well at 28-35°C. C. indica strains were evaluated for their mycelial growth, extracellular enzymes producing capability and their yield potential. Different characteristic of nine strains of Calocybe indica Ci-01 to Ci-09 and a Macrocybe were grown on Potato Dextrose (PDA), Complete Yeast Extract (CYM), Mushroom Minimal Media (MMM) and their broth at 30±2°C. After 10 days, maximum radial growth was observed on PDA in Ci-06 (87.6 mm) and Ci-09 (87 mm) strain of C. indica. The maximum biomass was recorded in CYM in Ci-06 (16.1g/l) and Ci-07 (15.5 g/l). The maximum linear growth on wheat straw after 10 days was observed in Ci-07 (63mm) and Ci-09 (66mm). In wheat straw, the maximum biological efficiency estimated from the harvested yield (kg/q dry straw) was observed in the strain Ci-06 (57.3%). Two strains Ci-07 (48.5%) and Ci-09 (45.7%) were found to give yield at par with each other. Maximum number of fruiting bodies were recorded in the strain Ci-06 (1552 no./q dry straw). In culture filtrate endoglucanase enzyme activity range between 1.91-4.42 U/mg, endoxylanase activity ranged between 2.73-4.76 U/mg, laccase activity ranged between 6.94-10.2 U/mg. During spawn run, endoglucanase and endoxylanase enzyme activity ranged between 0.58-1.03 U/mg and 0.74 -2.11 U/mg, laccase activity was ranged between 1.51-4.54 U/mg. During pinhead, endoglucanase and endoxylanase activity ranged between 0.61-1.59 U/mg and 1.57-2.96 U/mg, whereas laccase activity range between 6.53-10.6 U/mg. In fruiting bodies, endoglucanase and endoxylanase activity of the strains ranged between 0.94-1.88 U/mg and 0.93-2.36 U/mg, laccase activity ranged between 5.43-9.21 U/mg. A positive correlation of yield with biomass and endoglucanase has been observed in C. indica strains Ci-03, Ci-06, Ci-07 and Ci-09.
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