Effect Of Some Additives On Cryopreservation Of Buffalo Bull Semen Using Tris-Yolk-Glycerol
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Date
1990
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
Total of fifty ejaculates (10 from each of the five fertile buffalo bulls) with initial motility 70 % were processed for deep-freezing in liquid nitrogen (- 19 6 C) in six extenders viz. Tris- yolk- glycerol (T 1), Tris-yolk glycerol-chloroquine diphosphate ( T 2 ), Tris- yolk-glycerolchlorpromazine hydrochloride (T3), Tris-yolk-glycerolcaffeine(T), Tris -yolk-glycerol -cysteine hydrochloride (T5) and Tris-yolk-glycerol- ascorbic acid (T6). Chloroquine or as corbic acid addition resulted in better freezability (P< 0.05) of semen at all stages (Post extension, Postequilibration and Post freoethaw) in terms of higher percentage of motile sperms, live sperms and intact acros somes and low percentage of head and tail abn or malities. Suppli-mentation cf chlor promazine hydrochloride pr oved to be advantageous for maintenance of sperm morphology at all stages of deep-freezing but sperm motility and livability was significantly (P.< 0.05) lower as compared to TI(Control) atpost freeze the wastage. Caffeine addition (7 m M) was detri-
mental to spermatozoa in terms of motility and livability but significant (P< 0.05 ) pronounced effect was observed at post freeze thaw stage. Morphology of sperms was adversely affected at all stages of freeze preservation. Cysteine hydrochloride modified Tris-yolk- glycerol did shows some beneficial effect in freezability of semen but significant (P< 0.05) improvement was seen in terms of live spermatozoa
(%) at post freeze thaw stage and percentage of intact acrosomes at post equilibration and post-freeze thaw stages.
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