Morpho-physiological and molecular characterization of drought pyramided PR121 variety of rice (Oryza sativa L.)
| dc.contributor.advisor | Rupinder Kaur | |
| dc.contributor.advisor | Rupinder Kaur | |
| dc.contributor.author | Parminder Singh | |
| dc.contributor.author | Parminder Singh | |
| dc.date.accessioned | 2019-03-08T04:41:36Z | |
| dc.date.available | 2019-03-08T04:41:36Z | |
| dc.date.issued | 2019 | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Punjab is a major rice producing state of India. It is facing the problem of receding water table due to intensive cultivation. Thus, attempts were made to improve the water use efficiency of the predominantly grown high yielding variety PR121 by introducing two drought QTLs qDTY1.1 and qDTY3.1 from donor parent IR96321-1447-561-B-1 using marker assisted selection (MAS). A set of 72 BC1F4 lines in the background of PR121 derived from this cross was evaluated for various morpho-physiological traits under reproductive stage drought stress as well as non stress conditions during Kharif 2017. Significant genotypic variation was observed for days to 50% flowering, plant height, ear bearing tillers/m2, spikelet fertility, thousand grain weight, proline content, SPAD reading and yield/plant under both the environments.Most of the lines early under stress conditions. About 10 lines had similar days to flowering under both stress and non stress conditions. The plant height, ear bearing tillers/m2, spikelet fertility and grain yield/ plant were significantly reduced under water stress conditions while the proline content increased. The progeny lines 1680, 1690, 1883, 1922 and 1975 had good yield under both environments. The line 1883 had desirable maturity, high yield, drought tolerance traits as well as good milling quality. The 72 BC1F4 lines were subjected to foreground and background selection. Foreground selection showed presence of qDTY1.1, qDTY3.1 and both QTLs in 36, 15 and 21 lines respectively. Background selection was carried out using 56 polymorphic SSR markers present on 12 chromosomes. Maximum recurrent parent genome was present in the line 1607. Among the five best lines selected on the of morpho-physiological traits, the line 1922 showed maximum background recovery of 84.5% and line 1975 showed minimum background recovery of 58.8%. These lines also showed bacterial blight resistance against pathotype VII similar to the recurrent parent. These lines with drought tolerance will help to improve the water use efficiency of PR121 and also serve as a source to improve cultivars in the future. | en_US |
| dc.description.abstract | Punjab is a major rice producing state of India. It is facing the problem of receding water table due to intensive cultivation. Thus, attempts were made to improve the water use efficiency of the predominantly grown high yielding variety PR121 by introducing two drought QTLs qDTY1.1 and qDTY3.1 from donor parent IR96321-1447-561-B-1 using marker assisted selection (MAS). A set of 72 BC1F4 lines in the background of PR121 derived from this cross was evaluated for various morpho-physiological traits under reproductive stage drought stress as well as non stress conditions during Kharif 2017. Significant genotypic variation was observed for days to 50% flowering, plant height, ear bearing tillers/m2, spikelet fertility, thousand grain weight, proline content, SPAD reading and yield/plant under both the environments.Most of the lines early under stress conditions. About 10 lines had similar days to flowering under both stress and non stress conditions. The plant height, ear bearing tillers/m2, spikelet fertility and grain yield/ plant were significantly reduced under water stress conditions while the proline content increased. The progeny lines 1680, 1690, 1883, 1922 and 1975 had good yield under both environments. The line 1883 had desirable maturity, high yield, drought tolerance traits as well as good milling quality. The 72 BC1F4 lines were subjected to foreground and background selection. Foreground selection showed presence of qDTY1.1, qDTY3.1 and both QTLs in 36, 15 and 21 lines respectively. Background selection was carried out using 56 polymorphic SSR markers present on 12 chromosomes. Maximum recurrent parent genome was present in the line 1607. Among the five best lines selected on the of morpho-physiological traits, the line 1922 showed maximum background recovery of 84.5% and line 1975 showed minimum background recovery of 58.8%. These lines also showed bacterial blight resistance against pathotype VII similar to the recurrent parent. These lines with drought tolerance will help to improve the water use efficiency of PR121 and also serve as a source to improve cultivars in the future. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810097732 | |
| dc.keywords | Rice, drought, backcross, reproductive stage, yield, Marker Assisted Selection. | en_US |
| dc.keywords | Rice, drought, backcross, reproductive stage, yield, Marker Assisted Selection. | en_US |
| dc.language.iso | en | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 53 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Morpho-physiological and molecular characterization of drought pyramided PR121 variety of rice (Oryza sativa L.) | en_US |
| dc.research.problem | Morpho-physiological and molecular characterization of drought pyramided PR121 variety of rice (Oryza sativa L.) | en_US |
| dc.sub | Genetics and Plant Breeding | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Morpho-physiological and molecular characterization of drought pyramided PR121 variety of rice (Oryza sativa L.) | en_US |
| dc.title | Morpho-physiological and molecular characterization of drought pyramided PR121 variety of rice (Oryza sativa L.) | en_US |
| dc.type | Thesis | en_US |